出生前后双酚A暴露对小鼠不同时期血清白介素-17白介素-23的影响  

Effects of prenatal and postnatal exposure to bisphenol A on serum IL-17 and IL-23 in serum of mice

作　　者：欧琪亮[1,2] 丁宋军[1] 李云[1] 房魏[1] 李美玲[1] 沈彤[1] 

机构地区：[1]安徽医科大学公共卫生学院 [2]中国人民解放军73061部队合肥干休所

出　　处：《安徽医学》2013年第5期530-532,共3页Anhui Medical Journal

摘　　要：目的研究出生前后高剂量双酚A(BPA)暴露对小鼠不同时期血清白介素-17(IL-17)和白介素-23(IL-23)水平的影响,初步探讨BPA发育免疫毒性机制。方法将确认妊娠的ICR母鼠随机分为空白对照组、溶剂对照组、BPA剂量组(高、中、低组分别为2.0、0.5和0.125 g/L BPA);母鼠自妊娠第0 d(GD0)至仔鼠断乳(出生后第21 d,PND21)经饮水暴露于BPA。分别于PND7、PND21和PND42取仔鼠外周血用ELISA检测IL-17和IL-23含量。结果在PND7和PND21时,随母鼠BPA暴露剂量的增加,仔鼠血清IL-17和IL-23含量逐渐上升,2.0 g/L BPA组与空白对照组和溶剂对照组相比,差异均有统计学意义(P值均<0.05)。在PND42时,各BPA暴露组仔鼠血清IL-17和IL-23含量与空白对照组和溶剂对照组差异均无统计学意义(P值均>0.05)。结论出生前后BPA暴露可能引起子代断乳前体内Th17细胞分泌的细胞因子含量升高。Objective To investigate the effects of prenatal and postnatal exposure to bisphenol A on serum IL-17 and IL-23 of mouse offspring,and explore the mechanism of developmental immune toxicity of bisphenol A.Methods Confirmed pregnancy ICR mice were randomly divided into blank control,solvent control,2.0 g / L BPA,0.5 g / L BPA and 0.125g / L BPA groups.Gestation mice were exposed to BPA through drinking water from gestation day 0（GD0） to offspring weaning（postnatal days 21,PND21）.At PND7,PND21 and PND42,pups peripheral blood was taken and IL-17 and IL-23 content in serum were assayed using ELISA.Results At PND7 and PND21,IL-17 and IL-23 content in serum of offspring increased gradually with exposure dose elevating in pregnancy mice,and the differences showed statistical significance between 2.0 g / L BPA group and blank control group or solvent control group（P ＆lt;0.05）.At PND42,IL-17 and IL-23 content in serum of offspring showed no significant difference between BPA exposed groups and blank control group or solvent control group（P＆gt; 0.05）.Conclusion Prenatal and postnatal BPA exposure may cause the increased cytokines secretion of Th17 cells before weaning in offspring.

关 键 词：双酚A 出生前后暴露 白介素-17 白介素-23 

分 类 号：R114[医药卫生—卫生毒理学]