玻璃化冷冻与慢速冷冻对微量精子活动力和DNA完整性的影响  

Effects of vitrification and standard slow freezing method on motility and DNA integrity of small numbers of human spermatozoa

作　　者：张静静[1] 章志国[1] 贺小进[1] 吴欢[1] 曹云霞[1] 

机构地区：[1]安徽医科大学第一附属医院生殖中心,生殖与遗传研究所,合肥230022

出　　处：《安徽医学》2013年第5期533-536,共4页Anhui Medical Journal

基　　金：国家自然基金项目(项目编号:30973197);安徽省十二五科技攻关计划(项目编号:11010402166)

摘　　要：目的比较玻璃化冷冻与慢速冷冻微量精子对精子活力和DNA完整性的影响。方法取70份正常精液标本上游处理后,每份标本分别进行慢速冷冻和玻璃化冷冻。观察冷冻前及2组冷冻组复苏后精子活动率和DNA碎片指数(DFI),比较两种方法的冷冻效果。结果冷冻前精子活动率和DNA碎片指数分别为(93.24±2.26)%和(6.70±5.78)%;玻璃化冷冻复苏后精子活动率及DFI分别为(63.07±6.69)%和(9.26±7.00)%;慢速冷冻复苏后精子活动率及DFI分别为(63.99±5.88)%和(9.58±6.89)%。两种方法复苏后精子活动率均较冷冻前显著性降低(P<0.05),两种方法复苏后精子DFI均较冷冻前显著性升高(P<0.05),两种冷冻方法复苏后精子活动率和精子DFI差异无统计学意义(P>0.05)。结论玻璃化冷冻和慢速冷冻均导致精子活动率下降及DNA损伤,但两种冷冻方法之间无显著差异。Objective To compare the efficiency of vitrification and standard slow freezing method on motility and DNA integrity of small numbers of human spermatozoa.Methods Seventy semen samples were washed by routine swim-up,each sample was divided into two parts for vitrification and standard slow freezing respectively.Motility and DNA fragmentation index（DFI） of the fresh spermatozoa,and thawed spermatozoa after vitrification and standard slow freezing were detected respectively.Results The motility and DFI of he fresh spermatozoa were（93.24±2.26）% and（6.70±5.78）%;the post-thaw motility and DFI of standard slow freezing method group were（63.99±5.88）%and（9.58±6.89）%）;the post-thaw motility and DFI of vitrification group were（63.07±6.69）%and（9.26±7.00）%.The motility in two cryopreservation methods were significantly lower than those before cryopreservation（P＆lt;0.05）.There was no significant difference in motility between two cryopreservation methods（P＆gt;0.05）.The DFI in two cryopreservation methods were significantly higher than those before cryopreservation（P＆lt;0.05）.There was no significant difference in DFI between two cryopreservation methods（P＆gt;0.05）.Conclusion Vitrification and standard slow freezing method all lead to spermatozoa motility decline and DNA damage,but there is no significant difference between two cryopreservation methods.

关 键 词：精子冷冻 玻璃化 DNA碎片指数 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]