HPLC法同时测定北柴胡中柴胡皂苷a、b_2、c、d、f的含量  

Simultaneous Determination of Saikosaponins a、b_2 、c、d、f in Radix Bupleuri by HPLC

作　　者：邓平[1] 张国松[2] 罗晓健[3] 王跃生[3] 

机构地区：[1]南昌航空大学,南昌330034 [2]北京中医药大学,北京100102 [3]江西中医学院,南昌330004

出　　处：《江西中医学院学报》2013年第4期46-49,共4页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：重大新药创制专项(项目编号:2009ZX09103)

摘　　要：目的:建立高效液相色谱法-紫外(HPLC-UV)同时测定北柴胡中柴胡皂苷a、b2、c、d、f的含量方法。方法:采用Hypersil ODS2C18色谱柱(4.6mm×250mm,5μm),以乙腈-水为流动相梯度洗脱(0min:33:67;20min:40:60;35min:53:47;40min:60:40;45min:33:67),流速:1mL/min,柱温:室温,紫外检测器,检测波长:210nm。结果:柴胡皂苷a、b2、c、d、f分别在2.51-10.54,1.64-6.56,2.02-8.08,2.06-8.24,2.07-8.28μg的范围内线性关系良好(r分别为0.9970,0.9990,0.9991,0.9978,0.9998);平均回收率分别为99.8%,98.9%,100.2%,98.6%,99.3%。结论:该方法简便、快捷、重复性好,可用于柴胡药材的质量检测。Objective： to establish an HPLC simultaneous determination of saikosaponins a, b2, c, d, f in Radix Bupleuri. Methods： The analysis was performed on hypersil ODS2 C18 column （4.6mm×250mm,5μm） at the room temperature with a gradient program of acetonitrile-water （0rain： 33： 67; 20min： 40： 60; 35min： 53： 47; 40min： 60： 40; 45min： 33： 67） asthemobile phase and a DAD as the detector, The detector wavelength is 210 nm, The flow is lmL/min. Results： Saikosaponins a. b2, c. d, f were linear over the range of 2.51-10.54, 1.64-6.56, 2.02-8.08, 2.06-8.24, 2.07-8.28μg （R was 0.9970, 0.9990, 0.9991, 0.9978, 0.9998, respectively） .The average recoveries were 99.8%, 98.9%, 100.2%, 98.6%, 99.3% for Saikosaponins a. b2, c. d, f, respectively. Conclusion： This method is sample, rapid, reliable and reproducible. It can be used for the quality control of Radix Bupleuri.

关 键 词：柴胡 柴胡皂苷 高效液相 

分 类 号：R284[医药卫生—中药学]