水蛭提取物对人白血病HL60细胞体外抑制作用研究  

Study on Inhibitory Functions of Leech Extract on HL60 Cells in Vitro Experiment

作　　者：肖移生[1] 廖夫生[1] 赵志冬[1] 左爱仁[1] 朱大诚[1] 

机构地区：[1]江西中医学院,南昌330004

出　　处：《江西中医学院学报》2013年第4期63-66,共4页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：江西中医学院2012年校级课题(项目编号:2012ZR005)

摘　　要：目的:研究水蛭提取物对人白血病HL-60细胞的体外抑制作用。方法:用液氮快速冻融法制备水蛭提取物,将不同浓度提取物作用HL-60细胞,观察提取物对HL60生长与增殖的影响、诱导调亡效应、并计算出水蛭提取物作用HL-60的半数抑制浓度(IC50)。结果:浓度高于0.1mg/mL的水蛭提取物对HL60有抑制作用,且呈时间和剂量依赖性;水蛭提取物作用HL60细胞48h的IC50为1.4mg/mL;另外水蛭提取物也具有诱导HL60细胞凋亡作用。结论:水蛭提取物可抑制HL60细胞增殖并诱导调亡。Objective： To observe the inhibitory functions of Leech Extract on human leukemic HL60 cells. Methods： HL60 cells were treated with different concentrations of leech extracts which were extracted by method of freeze-thawing with liquid nitrogen, then examine the grouth, proliferation, and apoptosis of HL60 cells, and calculate IC50 concentration of Leech Extract on HL60 cells. Results： Above the 0.1 mg/ml drug concentrations of Leech Extract has a obvious inhibition on proliferation of HL60 which in dose and time-dependent mannaer. The IC50 concentration of Leech Extract on HL60 cells is 1.4rag/m1. In addition, different concentrations of Leech Extract can induce cell apoptosis of HL60 significantly. Conclusion： Leech Extract can inhibit the growth and proliferation of HL60 cell strongly, and can induce cell apoptosis of HL60.

关 键 词：水蛭提取物 HL60 增殖 调亡 

分 类 号：R285.5[医药卫生—中药学]