HPLC测定姜黄素混悬剂中姜黄素的含量  

Determination of Curcumin Suspensions by HPLC

作　　者：李梦婷[1,2,3] 纪宏宇[1,2,3] 唐景玲[2,3] 任金妹[1,2,3] 崔超[1,2,3] 刘红梅[1,2,3] 吴琳华[1,2,3] 

机构地区：[1]哈尔滨医科大学附属第二医院药学部,哈尔滨150086 [2]哈尔滨医科大学药学院 [3]黑龙江省高校重点实验室

出　　处：《中国药师》2013年第11期1648-1650,共3页China Pharmacist

基　　金：黑龙江省青年科学基金项目(编号:QC2011C104);黑龙江省教育厅科学技术研究项目(编号:11551274)

摘　　要：目的:建立高效液相色谱法测定姜黄素混悬剂中姜黄素的含量。方法:采用Diamonsil^(TM)C_(18)(200 mm×4.6 mm,5μm),流动相:乙腈-甲醇-水(35:45:20),检测波长:430 nm,柱温:25℃,流速:1.0 ml·min^(-1),进样量20 ml。结果:姜黄素在0.05~5.00μg·ml^(-1)范围内线性关系良好(r=0.999 8),平均回收率为98.6%,RSD为1.3%(n=9)。结论:本法简单准确、重复性好,可用于姜黄素混悬剂中姜黄素的含量测定。Objective： To establish an HPLC method for the determination of curcumin in curcuma suspensions. Method： The de- termination was carried out on a DiamonsilTM C18 column （200 mm~ 4.6 mm, 5 m） with acetonitrile-methanol-water （35：45：20） as the mobile phase, the detection wavelength was at 430 nm, the column temperature was 25~C, the flow rate was 1.0 ml ~ rain-1 and the sample size was 20 ~1. Result： A good linear relationship was shown within the range of 0.05-5.00 g ml - t （ r = 0. 999 8）, the recovery was 98.6% and RSD was 1.3% （ n = 9）. Conclusion ： The method is simple and reliable with good reproducibility, and can be used in the determination of curcuma suspensions.

关 键 词：高效液相色谱法 姜黄素 混悬剂 含量测定 

分 类 号：R927.2[医药卫生—药学]