酸感受离子通道及钠氢泵基因表达在痫性发作中的作用研究  

Effect of acid senor ion channels （ASICs） and Na＋/H＋ exchanger gene expression in seizure attack

作　　者：陈煦明 梁静静[1] 潘松青[1] 卢祖能[1] 肖哲曼[1] 

机构地区：[1]武汉大学人民医院神经内科,430060

出　　处：《神经疾病与精神卫生》2013年第5期440-443,共4页Journal of Neuroscience and Mental Health

基　　金：国家自然科学基金资助项目（30900459）;国家教育部新教师基金资助项目（200804861046）;武汉大学青年教师资助项目（3082012）;湖北省教育厅科学技术研究计划指导性项目（B类）（B20090102）

摘　　要：目的探讨酸感受离子通道（ASICs）、钠氢泵（NHE）基因表达及其在痫性发作中的作用。方法联合运用脑电图及逆转录PCR方法，检测正常大鼠，匹鲁卡品致痫大鼠，阿米洛利海马ASIC1a，ASIC3及NHErnRNA表达。结果痫性发作1h即可观察到ASIC1a，ASIC3及NHEmRNA表达较正常大鼠增加，差异具统计学意义（P〈0．01）；痫性发作大鼠给予阿米洛利后，与对照组（痫性发作1h组）相比，ASIC1a，ASIC3mRNA表达差异有统计学意义（P〈0．01），NHEmRNA表达差异无统计学意义（P〉0．05）。结论痫性发作可致ASIC1a，ASIC3基因表达增加，抑制AslC1a，ASIC3基因可减弱痫性发作，而与时NHE的作用无关。Objective To investigate the effect of acid senor ion channels （ASICs） and Na＋/H＋ exchanger （NHE） gene expression in seizure attack. Methods Using electroencephalogram and RT-- PCR, to detect the correlation of seizure attack and ASICs/NHE gene expression in normal, seizure and amiloride--treated rats. Results Compared with the control group, ASIC la and ASIC 3 gene expres- sion increased in the rat hippocampal after 1 h and 2 h seizure attacking （P 〈 0. 01）. Compared with the lh seizure group, ASIC la and ASIC 3 gene expression decreased significantly in the rat hippocampal ap- plied with amiloride after seizure attacking （P 〈 0.01）. Compared with the control group, Na＋/H ＋ exchanger （NHE） gene expression increased in the rat hippocampal after 1 h and 2h seizure attacking （P 〈 0.01）. Compared with the lh seizure group, there＇s no difference of NHE gene expression in the rat hippocampal applied with amiloride after seizure attacking （P 〉 0.05）. Conclusions Seizure attac- king leads to elevation of ASIC la and ASIC 3 gene expression, amiloride decreased ASIC la and ASIC 3 gene expression which may inhibit and seizure attacking.

关 键 词：癫痫 酸感受离子通道 钠氢泵 阿米洛利 

分 类 号：R972[医药卫生—药品]