奥氮平诱导性肥胖大鼠血清和纹状体TNF—α表达变化  

Changes of serum and corpus striatum TNF-α in olanzapine--induced obese rats

作　　者：买买提热夏提·吐尔逊[1] 张荣[2] 桂学萍[1] 徐国正[1] 马晓洁[1] 

机构地区：[1]新疆乌鲁木齐市第四人民医院十三科,830002 [2]新疆医科大学基础医学院

出　　处：《神经疾病与精神卫生》2013年第5期473-476,共4页Journal of Neuroscience and Mental Health

基　　金：乌鲁木齐市科技局科技公关计划项目（G111810003）

摘　　要：目的观察奥氮平诱导性肥胖大鼠血清和大脑纹状体肿瘤坏死因子-α（TNF-α）含量的影响。方法40只SD雄性大鼠随机分为对照组和奥氮平组，各20只，前者采用普通饲料喂养，后者在普通饲粮喂养基础上灌胃奥氮平（1．2mg·kg^-1）4周建立奥氮平诱导肥胖大鼠。采用酶联免疫测定法测定血清中TNF-α的含量，生化比色法测定血清葡萄糖（FBS）含量，对脑组织采用HE染色观察形态学变化，采用免疫组织化学方法观察两组大鼠脑组织中TNF-α的表达。结果与对照组比较，奥氮平组大鼠的体重、血糖、血脂和血清TNF-α水平和纹状体TNF-α蛋白表达均有不同程度的升高，差异有统计学意义（P〈0．01）。结论奥氮平可以导致大鼠体重增加，糖脂代谢紊乱，血清及纹状体TNF-α升高，通过调节TNF-α异常变化可能改善奥氮平诱导肥胖大鼠中枢摄食活动。Objective To evaluate the effect of olanzapine on serum tumor necrosis factor α （TNF -α） contents and corpus striatum TNF-α protein expression in obese rats induced by olanzapine. Meth- ods 20 rats fed with ordinary fodder were used as normal control group, olanzapine group contained 20 rats fed with olanzapine（1.2 mg · kg-1） and ordinary fodder for 4 weeks. Successful experimental obese rat model induced by olanzapine was established after 4 weeks of treatment. Serum TNF-α contents were measured by Elisa. TNF-α protein expression were investigated in corpus striatum with immuno- histochemistry methods. Serum glucose contents were detected using biochemical colorimetric method. Blood lipid contents were determined with automatic biochemical analyzer. HE staining was used to as- sess brain morphology. Results blood glucose , blood lipid and serum TNF-α level and corpus striatum TNF-α protein expression were higher in olanzapine group than those in model control group （P 〈0.01）. Conclusions Olanzapine can induce weight gaining, metabolic disturbance of blood glucose, blood lipid, and up--regulation of serum TNF and corpus striatum TNF-α in rat. There is a potential to influence central feeding activity in olanzapine--induced obese rats by interfering TNF-α abnormal changes.

关 键 词：奥氮平 大鼠 肿瘤坏死因子-α 纹状体 

分 类 号：R442.2[医药卫生—诊断学]