Differences in the gene expressive quantities of carbonic anhydrase and cysteine synthase in the weathering of potassium-bearing minerals by Aspergillus niger  

作　　者：SUN LeiLei XIAO LeiLei XIAO Bo WANG WeiYing PAN Chen WANG ShiJie LIAN Bin 

机构地区：[1]Jiangsu Key Laboratory for Microbes and Functional Genomics,Jiangsu Engineering and Technology Research Center for Microbiology,College of Life Science,Nanjing Normal University [2]State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences

出　　处：《Science China Earth Sciences》2013年第12期2135-2140,共6页中国科学（地球科学英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.41173091);National Key Basic Research Program of China(Grant No.2013CB956702);the Priority Academic Program Development of Jiangsu Higher Education Institutions

摘　　要：We investigated the differences in the gene expression of carbonic anhydrase （CA） and cysteine synthase （CysM） between two weathering conditions, with either soluble potassium or insoluble potassium. We cultured a strain of A. niger by adopting a variant Czapek medium （using NazHPO4 as a substitute for KzHPO4） in two groups, Group A （containing silicate minerals bearing potassium but without KC1） and Group B （with KCI） . We extracted the mRNAs of CA and CysM from these two groups and performed real-time quantitative polymerase chain reactions （RT-qPCR）. We constructed relative standard curves by using glyceraldehyde-3-phosphate dehydrogenase （GAPDH） as the reference to confirm a consistent amplification effi- ciency of the target genes （CA and CysM） and the reference gene and quantified the gene expression of the targets in a relative manner. Our results showed that CA and CysM in Group A were upregulated for 1.7 times and 11.7 times, respectively, com- pared with those in Group B. Furthermore, we also analyzed some metabolic pathways and functions of the A. niger-induced weathering of potassium-bearing minerals, which involved the synthesizing of these two enzymes. Thus our work provides materials for further study of the roles of A. niger in the metabolic regulation during the weathering process of potassi- um-beating minerals.We investigated the differences in the gene expression of carbonic anhydrase(CA)and cysteine synthase(CysM)between two weathering conditions,with either soluble potassium or insoluble potassium.We cultured a strain of A.niger by adopting a variant Czapek medium(using Na2HPO4as a substitute for K2HPO4)in two groups,Group A(containing silicate minerals bearing potassium but without KCl)and Group B(with KCl).We extracted the mRNAs of CA and CysM from these two groups and performed real-time quantitative polymerase chain reactions(RT-qPCR).We constructed relative standard curves by using glyceraldehyde-3-phosphate dehydrogenase(GAPDH)as the reference to confirm a consistent amplification efficiency of the target genes(CA and CysM)and the reference gene and quantified the gene expression of the targets in a relative manner.Our results showed that CA and CysM in Group A were upregulated for 1.7 times and 11.7 times,respectively,compared with those in Group B.Furthermore,we also analyzed some metabolic pathways and functions of the A.niger-induced weathering of potassium-bearing minerals,which involved the synthesizing of these two enzymes.Thus our work provides materials for further study of the roles of A.niger in the metabolic regulation during the weathering process of potassium-bearing minerals.

关 键 词：Aspergillus niger potassium-bearing minerals potassium feldspar carbonic anhydrase cysteine synthase RT-qPCR 

分 类 号：R341[医药卫生—基础医学]