高效液相色谱法测定甘草远志合剂中甘草酸铵的含量  

The Content of Ammonium Glycyrrhizinate in Gancao Yuanzhi Heji in Detection with High Performance Liquid Chromatography

作　　者：郭琪[1] 程利娟[1,2] 雷虹[1] 申睿[1] 杨莹[1] 慕磊[1] 王彦宗[1] 

机构地区：[1]北京军区联勤部药品仪器检验所,北京100071 [2]河北北方学院药理教研室,河北张家口075000

出　　处：《解放军医药杂志》2013年第11期76-77,80,共3页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：军队中医药科研专项课题(NO.10ZYZ204)

摘　　要：目的建立高效液相色谱法测定甘草远志合剂中甘草酸铵的含量。方法色谱柱：Inertsil ODS．SP（4．6mm×250mm，5μm），流动相：乙腈-0．1％磷酸溶液（42：58），检测波长：252nm，流速：1．0ml／min，进样量：10μl，柱温：30℃。结果甘草酸铵在4．008～160．3μg／ml（r=0．9999）范围内，峰面积与其浓度呈良好的线性关系，平均回收率为100．79％，相对标准偏差为0．98％（n=6）。结论本方法准确、灵敏度高、重复性好、简便可行，可作为甘草远志合剂的质量控制方法。Objective To establish a high performance liquid chromatography （HPLC） to determine the content of ammonium glycyrrhizinate in Gancao yuanzhi heji. Methods The chromatographic column was Inertsil ODS-SP （4.6 mm × 250 mm, 5 μm） ; the mobile phase consisted of acetonitrile-0.1% phosphoric acid solution （42： 58） ; the detection wavelength was at 252 nm; the flow rate was 1.0 ml/min with 10μl of injection volume; the column tempera- ture was 30℃. Results There was good linear relationship between the peak area with the concentration when the am- monium glycyrrhizinate was in the range of 4. 008 - 160. 3 μg/ml （ r = 0. 999 9 ）. The average recovery rate was 100.79% , and relative standard deviation （RSD） was 0.98% （n = 6）. Conclusion The method is accurate and con- venient with high sensitivity and good repetitiveness, and can be used in the quality control of Gancao yuanzhi heji.

关 键 词：甘草远志合剂 甘草酸铵 色谱法 高效液相 

分 类 号：R931.5[医药卫生—生药学]