碳酸钙联合阿法骨化醇对去卵巢大鼠骨质疏松防治作用  

The study of the preventive effect of the combined therapy of calcarea carbonica and alfacalcidol on osteoporosis in ovariectomized rats

作　　者：唐祝奇[1] 秦宁[1] 刘春[2] 朱晓晖[1] 印彤[1] 崔世维[1] 

机构地区：[1]南通大学附属医院内分泌科,江苏226001 [2]南通大学实验动物中心

出　　处：《交通医学》2013年第5期439-441,共3页Medical Journal of Communications

摘　　要：目的:探讨碳酸钙联合阿法骨化醇防治去卵巢大鼠骨质疏松的效果。方法:清洁级5月龄Wistar雌性大鼠40只,随机分为假手术组、模型组、碳酸钙组、阿法骨化醇组、碳酸钙+阿法骨化醇联合组(联合组),每组8只,手术切除卵巢进行骨质疏松造模,术后分别予蒸馏水、碳酸钙、阿法骨化醇和碳酸钙+阿法骨化醇灌胃,连续给药12w。监测体重,观察治疗后子宫指数、雌激素、骨密度、骨钙素、抗酒石酸酸性磷酸酶的变化。结果:与假手术组相比,模型组体重增加,雌激素下降,子宫指数及骨密度下降,造模成功。碳酸钙组及阿法骨化醇组与模型组相比,骨矿物质密度(bone mineral density,BMD)、骨钙素(bone gla protein,BGP)、血清抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,TRAP)改善,碳酸钙+阿法骨化醇组改善更显著。结论:碳酸钙和阿法骨化醇可抑制骨吸收和提高骨密度,防治去卵巢大鼠骨质疏松,两者联用有协同作用。Objective： To study the preventive effect of the combined therapy of calcarea carbonica and alfacalcidol on osteoporosis in ovariectomized rats. Methods：Forty female Wistar rats were randomly divided into five groups： Group of sham operation （n=8）, Group of model （n=8）, Group of calcarea carbonica （n=8）, Group of alfacalcidol （n=8）, and Group of calcarea carbonica＋alfacalcidol（n=8）, and they were given by intragastric administration with pure water, calcarea carbon-ica, alfacalcidol, and calcarea carbonica ＋ alfacalcidol, respectively for 12 ws after ovariectomization or sham operation. The changes of the indexes of the uteruses, the leves of serum estrogen, bone mineral density（BMD）, serum bone gla protein （BGP）, and serum tartrate-resistant acid phosphatase （TRAP） were observed. Results：Compared with Group of sham opera-tion, the body weight increased, while the levels of estrogen, indexes of the uteruses and BMD decreased significantly in Group of model（P〈0.05）. The levels of BMD, BGP, and TRAP improved significantly in Group of calcarea carbonica, Group of alfacalcidol, and especially in Group of calcarea carbonica＋alfacalcidol, compared with Group of model（P〈0.05）. Con-clusions： Treatment with calcarea carbonica and alfacalcidol can prevent the development of osteoporosis by increase in bone formation with reduction in bone resorption in ovariectomized rats, and the synergy effects were observed in the com-bined therapy.

关 键 词：骨质疏松症 去卵巢大鼠 碳酸钙 阿法骨化醇 

分 类 号：R681[医药卫生—骨科学]