羊水、脐带和骨髓来源间充质干细胞对淋巴细胞增殖的抑制效果比较  

Inhibitory effect of amniotic fluid-derived mesenchymal stem cells versus umbilical cord blood mesenchymal stem cells and bone marrow Mesenchymal stem cells on lymphocyte proliferation

作　　者：朱海鹏[1] 彭亮[2] 王培培[2] 杜巍[1] 殷思纯[1] 李发武[1] 

机构地区：[1]东莞市人民医院感染科,东莞523059 [2]中山大学附属第三医院传染科,广州510630

出　　处：《新医学》2013年第11期743-747,共5页Journal of New Medicine

基　　金：东莞市医疗卫生单位科技计划项目(200910515022)

摘　　要：目的比较羊水来源间充质干细胞(AF-MSC)、脐带来源间充质干细胞(UC-MSC)和骨髓来源间充质干细胞(BM-MSC)对植物血凝素诱导的淋巴细胞增殖的抑制作用。方法从妊娠中期羊水、新生儿脱落脐带、正常成人骨髓中分离获取原代AF-MSC、UC-MSC和BM-MSC。观察形态并采用流式细胞术检测表面分子。取传代培养的第3代的AF-MSC、UC-MSC和BM-MSC体外诱导培养后,观察体外诱导成骨成脂多向分化情况。将不同细胞浓度的AF-MSC,UC-MSC和BM-MSC分别加入到植物血凝素诱导的外周血淋巴细胞增殖体系中,比较3种不同来源的MSC对淋巴细胞增殖的调控作用。结果分离培养的AF-MSC,UC-MSC和BM-MSC分别表达MSC特异性表面分子CD29、CD70、CD90,不表达造血干细胞表面分子CD45。体外传代培养的AF-MSC、UC-MSC和BM-MSC对淋巴细胞增殖的抑制中,当淋巴细胞∶MSC为1∶1、1∶10、1∶50、1∶100时,对淋巴细胞增殖的抑制率AF-MSC最强,BM-MSC最弱。结论 AF-MSC、UC-MSC和BM-MSC均具有抑制植物血凝素诱导淋巴细胞增殖的作用,其中以AF-MSC抑制效果最强,BM-MSC最弱,其抑制效果与MSC数量有关。Objective To compare the inhibitory effect of amniotic fluid-derived mesenchymal stem cells （ AF-MSC） , umbilical cord blood mesenchymal stem cells （ UC-MSC ） and bone marrow Mesenchymal stem cells （ BM-MSC） on phytohemagglutinin （ PHA） -induced lymphocyte proliferation.Methods Human mesenchymal stem cells from the second trimester amniotic fluid , umbilical cord and normal adult bone mar-row, flow cytometry were extracted and detected the surface molecules of MSC.Induce the MSCs differentiate into osteogenic like and adipogenic like cells in virto.AF-MSC, UC-MSC and BM-MSC were added to PHA-in-duced lymphocyte proliferation system at different ratio , compared inhibitory effect of three different sources-de-rived mesenchymal stem cells on lymphocyte proliferation.Results Mesenchymal stem cell-specific surface molecules CD29, CD70 and CD90 were expressed by isolated AF-MSC, UC-MSC and BM-MSC, respectively.However, hematopoietic stem cell surface molecule CD 45 was not expressed.In vitro cultures of AF-MSC, UC-MSC and BM-MSC on lymphocyte proliferation inhibition , when lymphocytes： mesenchymal stem cells 1： 1, 1： 10, 1： 50, 1： 100, the right lymphocyte proliferation inhibition rate AF-MSC strongest, BM-MSC wea-kest.Whenever the of ratio of lymphocytes and mesenchymal stem cells are 1： 1, 1： 10, 1： 50, 1： 100, the lymphocyte proliferation inhibition of AF-MSC is strongest , and BM-MSC is weakest.Conclusion AF-MSC, UC-MSC and BM-MSC can inhibit PHA-induced lymphocyte proliferation , in which the AF-MSC is the strongest and BM-MSC is the weakest.Further study indicates that the effect was associated with the number of ratio of MSC to LC.

关 键 词：羊水 脐带 骨髓 间充质干细胞 免疫抑制 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]