qRT-PCR检测中通过β-ActinCt值预测检测因子Ct值  

Prediction of Factor Ct values in qRT-PCR detection Through β-Actin Ct

作　　者：孟馥芬[1] 夏庆杰[2] 维拉[1] 林娜[3] 徐杨[2] 王廷华[2] 

机构地区：[1]新疆医科大学附属肿瘤医院麻醉科,新疆乌鲁木齐830011 [2]四川大学华西医院转化神经科学中心,四川成都610041 [3]昆明医科大学神经科学研究所,云南昆明650031

出　　处：《新疆医学》2013年第11期4-8,共5页Xinjiang Medical Journal

基　　金：新疆维吾尔自治区青年自然科学基金资助。项目编号:2010211B13

摘　　要：目的:探讨qRT-PCR检测中β-Actin Ct值与检测因子Ct值检出率和相关性分析。方法:以32只成年SD大鼠脊髓为实验样本进行两次qRT-PCR检测,并分别测β-actin Ct值和PENK、CNTF、PDGF Ct值,随后进行独立样本T检验、卡方检验、一元线性回归分析。结果:两次qRT-PCR检测β-Actin Ct值无统计学差异(P>0.05);两次检测均显示β-actin Ct值小于20与β-actin Ct值大于20对其他因子的检出率有显著性影响(P<0.05);β-actin Ct值与检测因子Ct值有显著的线性相关性,线性回归方程为y=-0.983+0.708x(y:欲检测基因Ct值,x:实际测得β-actin Ct值)。结论:qRT-PCR检测中可通过β-Actin Ct值预测需检测因子的Ct值。Objective： To investigate the relationship between β -Actin Ct value and the detection factor Ct value in qRT-PCR. Methods： spinal cord samples of 32 adult SD rats were employed in the qRT-PCR detection, and we measured β -actin Ct values and PENK Ct value, CNTF Ct value, PDGF Ct value independently, followed by T-test, chi-square test, one yuan linear regression analysis. Results： the first and the second qRT-PCR detection of β -Actin Ct value was no significant difference （P〉0.05）; two tests have shown that β -actin Ct value of less than 20 and β -actin Ct values more than 20 was a key point of check rate for other factors （P〈0.05）; β -actin Ct value and detection factors＇ Ct values have a significant linear correlation, the linear regression equation was y = -0.983 ＋0.708 x （y： for detection of gene Ct value, x： the actually measured value of β -actin Ct）. Conclusion： via β -Actin Ct value prediction other factors Ct values in qRT-PCR detection.

关 键 词：实时定量反转录PCR Β-ACTIN CT值 脊髓 大鼠 

分 类 号：R446.61[医药卫生—诊断学]