自体脂肪颗粒预防椎板切除术后硬膜周围粘连的实验研究  

An experimental study on prevention of postlaminectomy adhesion by the use of autogenous fat particle

作　　者：刘瑞军[1] 周跃[1] 张文捷[1] 

机构地区：[1]第三军医大学附属新桥医院骨科,重庆市400037

出　　处：《中国脊柱脊髓杂志》2000年第6期344-347,共4页Chinese Journal of Spine and Spinal Cord

摘　　要：目的 :通过动物实验探讨预防硬膜外粘连的有效材料和方法。方法 :30只大白兔切除L1、L3、L5椎板造成 10×5mm硬脊膜裸露区后随机分为 5组 ,A组用纯化自体脂肪颗粒加碱性成纤维细胞生长因子 (bFGF)覆盖 ,B组用经胰岛素液浸泡的纯化自体脂肪颗粒覆盖 ,C组用未纯化自体脂肪颗粒覆盖 ,D组用 2 %透明质酸钠覆盖 ,E组不用任何间置物覆盖。术后 2、4、6、8、12周进行肉眼及光镜观察 ,并进行硬膜粘连程度分级。结果 :术后 12周时B、C、D、E组均形成了致密粘连 ,只A组无粘连发生。结论 :纯化自体脂肪颗粒加bFGF为预防椎管内粘连的最有效方法。Objective:To find out effective substances and methods for the prevention of peridural adhesion.Methods:Laminectomy was performed in lumbar 1,3 and 5 segment in 30 adult rabbits which were divided into 5 groups. A 10mm×5mm dura was exposed, and covered with purified autogenous fat particle mixed with bFGF in gourp A, with purified autogenous fat particle treated with insulin in group B, with unpurified autogenous fat particle in group C, with 2% SHA in group D, and uncovered in group E. The specimens were observed grossly and histologically at 2,4,6,8 and 12 weeks postoperatively.Result:Severe peridural adhesion were formed in group B,C,D,E after laminectomy, and no adhesion was formed in group A.Conclusion:Purified autogenous fat particle mixed with bFGF has the best effect in prevention peridural adhesion after laminectomy.

关 键 词：椎板切除术 硬膜 自体脂肪颗粒 透明质酸钠 

分 类 号：R392－33[医药卫生—免疫学]