指状青霉致突变性研究  

Study on the mutagenicity of Penicillium digitatum

作　　者：杨胜利[1] 赵国强[2] 张巧[3] 韩绍印[1] 于国强[1] 

机构地区：[1]河南医科大学食管癌研究室,河南郑州450052 [2]河南医科大学微生物与免疫学教研室,河南郑州450052 [3]河南医科大学劳动卫生教研室,河南郑州450052

出　　处：《河南医学研究》2000年第4期304-307,共4页Henan Medical Research

摘　　要：目的：研究指状青霉（Ｐｅｎｉｃｉｌｌｉｕｍ ｄｉｇｉｔａｔｕｍ）提取物的致突变性。方法：采用了细菌回复突变试验，小鼠骨髓 ＰＣＥ微核试验，大鼠肺及肝原代细胞程序外ＤＮＡ合成试验（ＵＤＳ）和大肠杆菌 Ｋ１２ ｉｎｆａ基因突变试验及 ｉｎｆＡ基因 序列测定和分析。结果：指状青霉提取物：①可明显地诱发大肠杆菌ＮＤ－１６０菌株回复突变；②可使小鼠骨髓 ＰＣＥ微核率极显著增高（Ｐ＜０．０１）；③可显著诱导大鼠肝原代细胞ＵＤＳ（Ｐ＜０．０５）及极显著诱导肺原代细胞ＵＤＳ （Ｐ＜０．０１）；④可诱发大肠杆菌Ｋ１２ ｉｎｆＡ基因ＤＮＡ序列中５个碱基位点突变，且其中１个位点的突变还可导致翻 译相应氨基酸的变异。结论：指状青霉对原核细胞和真核细胞，无论在体内或体外均有致突变活性。Objective: We studied the mutagenicity of the extract for Penicillium digitatum. Methods: Reverse mutation assay, micronucleus test, unscheduled DNA syntnesis (UDS) in the primary cells of the lung and liver of rat and E. coli K12 infA gene mutation assay(the sequences of mutated infA gene were detemined and then analyzed by computer). Results: ①The extract induced the reverse mutation of E. coli ND-160 strain;②The extract significantly did the increase of micronucleus rate of mouse bone marrow PCE(P < 0.01);③the extract could induce UDS in the primary cells of the lung and liver of rat,and UDS was particularly obvious in the primary cells of lung (P < 0.01 );④Five points mutation in nucleotide of infA gene were induced by the extract and one of them led to amino acid variation(Lys→Val). Conclusion: Penicillium digitatum could cause gene mutation.

关 键 词：指状青霉 细菌回复突变 微核 程序外DNA合成 

分 类 号：R117[医药卫生—公共卫生与预防医学]