黄芪多糖对RAW264.7巨噬细胞源性泡沫细胞胆固醇流出及PPARγ表达的影响  

Effects of Astragalus Polysaccharides on cholesterol efflux and the expression of PPARγ in RAW264.7 macrophage-derived foam cells

作　　者：燕李晨[1] 王庸晋[2] 郭静[3] 王金胜[3] 曹文君[3] 

机构地区：[1]山西医科大学附属第一临床医院长治医学院心血管研究所,太原市030001 [2]长治医学院心血管研究所长治医学院附属和平医院心血管病内科 [3]长治医学院心血管研究所

出　　处：《中国心血管病研究》2013年第11期901-905,I0005,共6页Chinese Journal of Cardiovascular Research

基　　金：山西省自然科学基金（2011011035-3）

摘　　要：目的 以RAW264.7巨噬细胞源性泡沫细胞为研究对象,观察不同浓度黄芪多糖（APS）对细胞内胆固醇流出及PPARγ表达的影响,探讨可能作用机制.方法 将RAW264.7细胞诱导分化为泡沫细胞,用油红O染色法鉴定.不同浓度APS作用泡沫细胞48 h后,液体闪烁计数仪检测胆固醇的流出率,并用RTPCR及ELISA测定PPARγ的基因及蛋白表达.结果 ①巨噬细胞经氧化型低密度脂蛋白（ox-LDL）诱导48 h后转化为泡沫细胞.②与对照组相比,不同浓度APS干预组泡沫细胞的胆固醇流出率上升（P＜0.01）,且在一定浓度范围内呈剂量依赖性.③与对照组相比,不同浓度APS干预组泡沫细胞内PPARγ的基因表达上调（P＜0.01）,且在一定浓度范围内呈剂量依赖性.④与对照组相比,20、50、100 mg/L浓度APS干预组泡沫细胞内PPARγ的蛋白表达增加（P＜0.01）.结论 ①经ox-LDL诱导后,巨噬细胞分化为泡沫细胞,细胞内脂质大量增加.②APS促进巨噬细胞内胆固醇流出,可能与上调细胞内PPARγ的表达有关.Objective To study the effects of Astragalus Polysaccharides （APS） on cholesterol efflux and the expression of PPART in RAW264.7 macrophage-derlved foam cells. Methods The mouse RAW264.7 cells were induced into foam cells, then we applied red oil O staining technique to appraise the foam cells. The RAW264.7 macrophage-derived foam cells were treated with APS at different concentrations. The cholesterel efflux of foam ceils was determined by Liquid Scintillation Counter; the expression of PPARγmRNA and protein was de- termined by RT-PCR and ELISA. Results （1）The macrophage cells were differentiated into foam cells after 48 hours by ox-LDL. （2）Compared with the control group, treatment of RAW264.7 macrephage-derived foam cells with different concentrations of APS increased the efflux of intracellular cholesterol in a dose-dependent manner （P〈 0.01）. （3）Compared with the control group, treatment of RAW 264.7 macrophage-derived foam cells with different concentrations of APS upregulated the expression of PPART mRNA in RAW264.7 ceils in a dose-dependent man- ner （P〈0.01）. （4）Compared with the control group, treatment of RAW264.7 macrophage-derived foam cells with 20 mg/L,50 rag/L,100 mg/L APS for 24 h resulted in an increase in the expression of PPART protein （P〈0.01）. Conclusion （1）The mouse RAW264.7 cells were differentiated into foam cells after 48 hours by ox-LDL, and theconcentration of intracellular lipid increased. （2）APS may enhance the efflux of intracellular cholesterol by upregu- lating PPARγexpression in RAW264.7 cells.

关 键 词：巨噬细胞 黄芪多糖 胆固醇流出 PPARΓ 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]