红外光谱内标法测定氯霉素片中氯霉素的含量  

Determination of Chloramphenicol in Chloramphenicol Tablets by Internal Standard Method of FTIR

作　　者：彭梦侠[1] 陈梓云[1] 

机构地区：[1]嘉应学院化学系,广东梅州514015

出　　处：《化工技术与开发》2013年第11期46-48,58,共4页Technology & Development of Chemical Industry

摘　　要：用红外光谱内标法（K3[Fe（CN）6]·6H2O为内标物）测定了氯霉素片中氯霉素的含量。实验表明，混合物红外光谱中氯霉素的测量峰（1686.4cm-1）和K3[Fe（CN）6]·6H2O的内标峰（2117.2cm-1）的峰高比（y=hi/hs）与两者质量比（x=mi/ms）有良好的线性关系，其线性回归方程为y=1.3127x＋0.5705，相关系数r＝0.9981。据此测得氯霉素片中氯霉素的含量为77.2%，相对标准偏差为1.8%，加标回收率分别为96.8%~103.8%，测定结果与药典规定的紫外分光光度法相近。该方法测量专属性强，无需分离提取，简便、快速、成本低，测定结果准确，特别适合快速批量检测，有应用价值。Potassium ferricyanide was added into chloramphenicol samples as internal reference and the mixtures were measured by Fou-rier transform infrared spectrophotometry （FT-IR）. The peak of chloramphenicol at 1686.4 cm-1 was chosen as quantitative peak and the one of potassium ferricyanide at 2117.2 cm-1 was chosen as inside mark quantitative peak. The ratio of two peak’s absorbance （y=hi/hs） had good linear relationship with their mass ratio （x=mi/ms）. The linear regression equation was y=1.3127x＋0. 5705 with the correlation coef-ficient r=0.9981. The contents of chloramphenicol in the samples of chloramphenicol tablets was 77.2%, the relatively standard deviation was 1.8%, the recoveries of the method was 96.8%~103.8%. This method was highly specific measure, especially for fast batch testing.

关 键 词：红外光谱内标法 氯霉素片 氯霉素 

分 类 号：O657.33[理学—分析化学]