铅铜复合胁迫对芥菜子叶抗性的影响  

Effect of combined lead and copper stress on resistance of Brassica juncea cotyledon

作　　者：邹文桐[1] 

机构地区：[1]福建师范大学福清分校生物与化学工程系,福建福清350300

出　　处：《西北农林科技大学学报（自然科学版）》2013年第11期191-195,208,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：福建省教育厅A类项目(JA12357)

摘　　要：【目的】研究不同质量浓度铅铜复合胁迫对芥菜子叶抗性指标的影响,为揭示重金属复合胁迫对植物抗性的影响机制提供参考。【方法】以超富集植物福州宽秆芥菜为试验材料,通过种子萌发试验,研究铅铜复合胁迫处理(对照(CK):0mg/L Pb(NO3)2+0mg/L CuCl2;处理1:100mg/L Pb(NO3)2+10mg/L CuCl2;处理2:200mg/L Pb(NO3)2+20mg/L CuCl2;处理3:300mg/L Pb(NO3)2+30mg/L CuCl2;处理4:500mg/L Pb(NO3)2+50mg/L CuCl2)对芥菜子叶中的可溶性蛋白质、可溶性糖、脯氨酸(Pro)、MDA含量和POD、SOD、CAT活性以及光合色素含量等指标的影响。【结果】随铅铜复合胁迫的加剧,芥菜子叶中的可溶性蛋白质和可溶性糖含量均呈先增后减的变化趋势,Pro和MDA含量较对照(CK)极显著增加,氧负离子产生速率呈增加趋势,POD活性呈先升后降趋势,SOD活性呈逐渐上升趋势,CAT活性呈逐渐降低趋势,芥菜子叶中的叶绿素a和叶绿素a+b呈先增加后减少趋势,而叶绿素b和类胡萝卜素含量则呈递减趋势。【结论】在受到铅铜复合胁迫时,芥菜的抗逆性降低,但同时也对较低质量浓度铅铜胁迫表现出一定的抗性。【Objective】This paper studied the effect of combined lead and copper stress on resistance of Brassica juncea cotyledon to reveal the mechanism of plant resistance to heavy metal stress.【Method】By seed sprouting test,taking the hyperaccumulation plant Fuzhou B.juncea as test material,the effects of combined lead and copper stress （control （CK）：0 mg/L Pb（NO3）2＋0 mg/L CuCl2,treatment 1：100 mg/L Pb（NO3）2＋10 mg/L CuCl2,treatment 2：200 mg/L Pb（NO3）2＋20 mg/L CuCl2,treatment 3：300 mg/L Pb（NO3）2＋30 mg/L CuCl2, and treatment 4： 500 mg/L Pb（NO3）2＋50 mg/L CuCl2）on soluble protein,soluble sugar,Pro,MDA contents and POD,SOD,and CAT activities and photosynthetic pigment contents of Brassica juncea cotyledon were studied.【Result】With the aggravation of combined lead and copper stress,soluble protein and soluble sugar,POD activity,chlorophyll a and chlorophyll a＋b of B.juncea cotyledon decreased after initial increase,Pro and MDA contents and O2- producing rate increased significantly compared to CK,SOD activity increased gradually,and CAT activity,chlorophyll b and carotenoid content decreased gradually.【Conclusion】Under combined lead and copper stress,stress resistance of B.juncea reduced,and the resistance of B.juncea to lead and copper in adversity was observed.

关 键 词：芥菜 铅 铜 抗性 

分 类 号：Q945.78[生物学—植物学] S637.201[农业科学—蔬菜学]