大孔树脂分离纯化酸枣仁总黄酮的工艺优选  

Optimization of Separation and Purification Technology for Total Flavonoids from Ziziphi Spinosae Semen by Macroporous Adsorption Resin

作　　者：王勇[1,2,3] 李洪福[1,2,3] 魏娜[1,2,3] 李永辉[1,2,3] 

机构地区：[1]海南医学院药学院,海口571199 [2]海南省热带药用植物研究开发重点实验室,海口571199 [3]海口市黎族医药重点实验室,海口571199

出　　处：《中国实验方剂学杂志》2013年第23期4-7,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金："十一五"国家科技支撑计划(2007BAI27B05);海南省自然科学基金项目(813192)

摘　　要：目的:优选大孔树脂分离纯化酸枣仁醇提物中总黄酮的工艺。方法:通过静态和动态吸附-洗脱试验比较6种不同型号大孔树脂对酸枣仁总黄酮的吸附和解吸性能,筛选最佳大孔树脂型号;以总黄酮质量浓度为指标,采用单因素试验考察药液质量浓度、吸附速率、上样体积、洗脱剂浓度、洗脱速率等因素对纯化工艺的影响。结果:大孔树脂分离纯化酸枣仁总黄酮的最佳工艺条件为选择SP-207型大孔树脂,上样液质量浓度0.5 g·mL-1,吸附速率1 BV·h-1,上样量5 BV,加70%乙醇4.5 BV以4 BV·h-1的速率洗脱;酸枣仁总黄酮纯度达65.47%。结论:该优选工艺稳定可行,适用于酸枣仁总黄酮的分离纯化。Objective： To optimize separation and purification technology of total flavonoids from ethanol extract of Ziziphi Spinosae Semen（ZSS） by macroporous adsorption resin. Method： Adsorption and desorption properties of six different types of macroporous resins for total flavonoids from ZSS were compared by static and dynamic adsorption and desorption tests to select optimum type of macroporous resin;With the content of total flavonoids as index,single factor tests were adopted to investigate adsorption and elution conditions of macroporous resin,such as the concentration of sample diluted,adsorption rate,eluent concentration and so on. Result： SP-207 macroporous resin was adopted,optimum separation and purification conditions for total flavonoids from ZSS were as follows：the concentration of sample solution 0.5 g mL-1,adsorption rate 1 BV h-1,sample volume 5 BV,eluted by 4.5 BV of 70% ethanol at a flow rate of 4 BV h-1;The content of total flavonoids from extract of ZSS was 65.47%. Conclusion： This optimized process was stable,feasible and suitable for separation and purification of total flavonoids from ZZS.

关 键 词：酸枣仁 总黄酮 大孔吸附树脂 分离纯化工艺 静态吸附-洗脱试验 动态吸附-洗脱试验 单因素试验 

分 类 号：R283.6[医药卫生—中药学]