正交试验优选黄连解毒汤的机器煎煮工艺  

Optimization of Machine Decoction Process for Huanglian Jiedu Decoction by Orthogonal Test

作　　者：蒋斌[1] 张星海[2,3] 唐樑[1] 沈夕坤[1] 李梅珍[1] 黄程[1] 朱晓燕[1] 王玳珠[1] 蔡宝昌[2,3] 

机构地区：[1]苏州市中医医院,江苏苏州215009 [2]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,南京210029 [3]江苏海异药业有限公司,南京210061

出　　处：《中国实验方剂学杂志》2013年第23期32-35,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2011年度苏州市科技发展计划项目(SYSD11165);江苏省科技支撑计划工业项目(BE2012011)

摘　　要：目的:优选黄连解毒汤的机器煎煮工艺。方法:以黄芩苷、小檗碱和浸出物含量的综合评分为指标,通过单因素试验和正交试验考察浸泡时间、煎煮时间、煎煮温度对黄连解毒汤的机器煎煮工艺的影响,并与传统煎煮工艺进行比较。采用HPLC测定指标成分含量,色谱条件为YMC-Pack ODS-A色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.2%磷酸水溶液(47∶53),检测波长250 nm。结果:黄连解毒汤的最佳机器煎煮工艺为浸泡时间10 min,煎煮温度105℃,煎煮时间30 min,明显优于传统煎煮工艺。结论:优化的机器煎煮工艺稳定可靠,评价指标可控,为中药汤剂的机器煎煮工艺制定提供实验依据。Objective： To optimize machine decoction process of Huanglian Jiedu decoction. Method： With composite score of baicalin,berberine and extractum contents as index,effects of soak time,decoction time and decoction temperature on machine decoction process were optimized by single factor tests and orthogonal test,and compared with traditional decoction process.Contents of index components were determined by HPLC,chromatographic conditions were as follows：YMC-Pack ODS-A column（4.6 mm250 mm,5 μm）,mobile phase of methanol-0.2% phosphoric acid（47：53）,detection wavelength 250 nm. Result： Optimal machine decoction process was as following：Soaked 10 minutes,decoction time 30 minutes,decoction temperature 105℃;it was better than traditional decoction process. Conclusion： Optimized machine decoction process was reasonable and feasible with controllable evaluation index,it could provide experimental basis for developing of machine decoction process of Chinese medicine decoctions.

关 键 词：黄连解毒汤 正交试验 高效液相色谱 机器煎煮工艺 差异性 单因素试验 

分 类 号：R283.6[医药卫生—中药学]