丹桃颗粒提取液的除杂工艺优选  

Optimization of Clarification Process of Dantao Granules

作　　者：吴建军[1] 刘新[1] 陈犁 

机构地区：[1]重庆医科大学药学院,重庆400042 [2]重庆市希尔安药业有限公司,重庆401520

出　　处：《中国实验方剂学杂志》2013年第23期36-39,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重大新药创制专项课题(2010zx09401-306-3-11)

摘　　要：目的:优选丹桃颗粒的壳聚糖絮凝剂澄清工艺,并与乙醇沉淀法、离心法进行比较。方法:以水浸出物收率、苦杏仁苷及芍药苷保留率为综合评价指标,选择壳聚糖加入量、絮凝时药液温度、药液pH为考察因素,通过正交试验优选丹桃颗粒的壳聚糖絮凝剂澄清工艺,并与乙醇沉淀法和离心法进行比较,确定最佳除杂工艺。结果:优选的壳聚糖絮凝剂澄清工艺为加入10%药液量的1%壳聚糖澄清剂,药液pH 6.0,50℃保温;苦杏仁苷、芍药苷保留率均>89%,水浸出固形物量约下降19%,整体优于乙醇沉淀法和离心法。结论:壳聚糖絮凝剂澄清法可作为丹桃颗粒提取液的除杂工艺,该工艺合理可行、稳定可靠,符合工业化生产要求。Objective： To optimize chitosan flocculant clarification process of Dantao granules and compare with ethanol precipitation method and centrifugation method. Method： With yield of water extract,retention rates of amygdalin and paeoniflorin as comprehensive evaluation index,orthogonal test was adopted to optimize chitosan flocculant clarification process of Dantao granules with chitosan dosage,flocculation liquid temperature and pH of liquid as factors,three clarification processes including ethanal precipitation method,adsorption clarification technique,high-speed centrifugation method were compared with nature sedimentation. Result： Optimized chitosan flocculant clarification process was as following：added 10% liquid volume of 1% chitosan,pH of liquid 6.0,flocculation liquid temperature 50℃;Water extract amount reduced to around 19%,retention rate of amygdalin and paeoniflorin were more than 89%,it was superior to ethanol precipitation method and centrifugation method. Conclusion： Chitosan flocculant clarification could be used as clarification process of Dantao granules,and this optimized technique was reasonable,stable and feasible for industrial production.

关 键 词：丹桃颗粒 除杂工艺 壳聚糖 正交试验 多指标加权综合评价法 

分 类 号：R283.6[医药卫生—中药学]