基质固相分散法提取牛蒡子中牛蒡子苷和牛蒡子苷元  

Extraction of Arctiin and Arctigenin from Arctii Fructus by Matrix Solid-Phase Dispersion

作　　者：高赛男[1] 杜雯雯[1] 李春香[1] 蒋蕾[1] 甄细娥[1] 李森[1] 

机构地区：[1]哈尔滨医科大学大庆校区药学院,黑龙江大庆163319

出　　处：《中国实验方剂学杂志》2013年第23期47-50,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省卫生厅基金项目(2011-234)

摘　　要：目的:探析基质固相分散法提取牛蒡子中牛蒡子苷和牛蒡子苷元的可行性。方法:以牛蒡子苷和牛蒡子苷元总提取量为评价指标,采用HPLC测定指标成分含量,通过单因素试验考察分散剂、洗脱剂、压实程度及洗脱流速对牛蒡子有效成分提取率的影响,并与甲醇超声提取法进行比较。结果:选取4倍量硅胶为分散剂,100倍量甲醇为洗脱剂,洗脱流速约40滴/min,压实程度为中度挤压;牛蒡子苷和牛蒡子苷元的总提取率达7.94%,略高于甲醇超声提取法的7.89%。结论:基质固相分散法操作简单、快速,可作为牛蒡子主要成分的提取方法,并为中药材有效成分的提取提供参考。Objective： To analysis feasibility of matrix solid-phase dispersion technology for extraction of arctiin and arctigenin from Arctii Fructus. Method： With total extraction amount of arctiin and arctigenin as index,which were determined by HPLC,effects of dispersants,eluents,compaction degree and elution flow rate on extraction rate of active ingredients from Arctii Fructus were investigated by single factor tests,and compared with methanol ultrasonic extraction technology. Result： Optimum matrix solid-phase dispersion technology was as following：with four times the amount of silica gel as dispersants,one hundred times the amount of methanol as eluents,compaction degree moderate extrusion,elution flow rate 40 drops/min. Total extraction ratio of arctiin and arctigenin was up to 7.94%,while it was 7.89% by methanol ultrasonic extraction technology. Conclusion： Matrix solid-phase dispersion method was simple and rapid,it could be used as a new method for extracting main ingredients from Arctii Fructus and provide a reference for extraction of active ingredients from Chinese herbal medicines.

关 键 词：基质固相分散法 牛蒡子 牛蒡子苷 牛蒡子苷元 高效液相色谱 单因素试验 

分 类 号：R285.5[医药卫生—中药学]