气质联用法鉴别柳叶润楠叶挥发油中化学成分  

Indentification of Chemical Components in Volatile Oil from Machilus salicina Hance by GC-MS

作　　者：牛燕燕[1] 钟琼芯[1] 陈光英[1] 韩倩倩[1] 陈伟娜[1] 范雪[1] 贾姗姗[1] 

机构地区：[1]海南师范大学化学与化工学院热带药用植物化学教育部重点实验室,海口571158

出　　处：《中国实验方剂学杂志》2013年第23期79-82,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国际科技合作项目(S2013ZR0211);国家自然科学基金项目(81160391)

摘　　要：目的： 鉴别柳叶润楠叶挥发油中化学成分并测定其相对含量。 方法： 采用水蒸气蒸馏法提取柳叶润楠新鲜叶中挥发油。运用GC-MS分析挥发油中化学成分并测定其相对含量，GC-MS条件为HP-FFAP石英毛细管柱（0.25 mm30 m，0.25 μm），起始温度40℃，以5℃min-1升温速率升温至200℃维持5 min，以8℃min-1升温速率升温至280℃维持至完成分析，载气为氦气（99.999%），柱流量1.0 mLmin-1，进样口温度250℃，分流比50∶1，EI电离源70 eV，离子源温度230℃，四极杆温度180℃，溶剂延迟2.5 min，扫描范围m/z 50~550。 结果： 挥发油提取率1.05%，共得到68种化合物，鉴定出55种（占挥发油总质量的77.10%），其中烯类化合物26个，醇类化合物12个，相对质量分数较高的组分为（Z-橙花叔醇（12.62%）、匙桉醇（7.48%）、喇叭茶醇（5.38%）。 结论： GC-MS可用于鉴别和测定挥发性成分含量，为柳叶润楠的开发利用提供实验依据。Objective： To identify chemical components in volatile oil from Machilus salicina Hance and determine their relative contents. Method： Volatile oil from fresh leaves of M. salicina Hance was extracted by hydrodistillation.GC-MS was adopted to analyze chemical constituents in volatile oil and determined their relative contents,mass spectrometric conditions were as follows：HP-FFAP quartz capillary column（0.25 mm30 m,0.25 μm）,the starting temperature 40℃,heated to 200℃ with 5℃ min-1,maintained 5 min,then with 8℃ min-1 heated to 280℃ until completion of analysis,helium as the carrier gas（99.999% ）,column flow rate 1.0 mL min-1,inlet temperature 250℃,split ratio 50：1,EI ionization source 70 eV,ion source temperature 230℃,quadrupole temperature 180℃,solvent delayed 2.5 min,scan range of m/z 50-550. Result： Yield of volatile oil was 1.05%,68 kinds of compounds were obtained and 55 kinds of compounds（accounted for 77.10% of total mass of volatile oil） were identified,26 of them was olefinic compounds,12 of them was alcohol compounds,the concentration of （Z）-nerolidol,spoon eudesmol and ledol were 12.62%,7.48% and 5.38%,respectively. Conclusion： GC-MS could be used to identify and determine content of volatile components,this study could provide experimental basis for development and utilization of M. salicina Hance.

关 键 词：柳叶润楠 挥发油 化学成分 GC-MS 匙桉醇 喇叭茶醇 

分 类 号：R284.1[医药卫生—中药学]