怀地黄药材HPLC指纹图谱研究  

HPLC Fingerprint of Rehmannia glutinosa

作　　者：王彦志[1] 张杰[1] 张华锋[1] 刘炯[1] 杨云[1] 冯卫生[1] 卫冰[1] 

机构地区：[1]河南中医学院药学院,郑州450046

出　　处：《中国实验方剂学杂志》2013年第23期83-86,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2011BAI06B00)

摘　　要：目的:建立怀地黄药材的HPLC指纹图谱,为科学评价及有效控制其质量提供可靠方法。方法:采用HPLC梯度洗脱法,对怀地黄药材进行测定,色谱条件,Dikma DiamonsilC18柱(4.6 mm×250 mm,5μm),乙腈-0.1%磷酸水系统梯度洗脱,流速1.0 mL·min-1,柱温35℃,检测波长205 nm。结果:标定了13个共有峰,建立了HPLC指纹图谱,该图谱的相关系数均在0.99以上。结论:该方法精确可靠,重复性好,可为控制怀地黄药材内在质量提供科学依据。Objective： To establish HPLC fingerprint of Rehmannia glutinosa to provide a reliable method for scientific evaluation and the effective control of its quality. Method： The fingerprints were obtained on a Dikma Diamonsil C18column （4.6 mm250 mm,5 μm） with the gradient elution solvent system composed of acetonitrile and 0.1% phosphoric acid. The flow rate was 1.0 mL min-1. The column temperature was maintained at 35℃,and the detection wavelength was set at 240 nm. Result： Thirteen common peaks were selected as the fingerprint peaks and the fingerprints were also evaluated by the Similarity Evaluation System for Chromatographic Fingerprint with the correlation coefficient above 0.99. Conclusion： This method with good precision and reproducibility is reliable for the quality control of R. glutinosa.

关 键 词：怀地黄 高效液相色谱法 指纹图谱 聚类分析 

分 类 号：R284.1[医药卫生—中药学]