益肾护骨方对废用性骨质疏松的改善作用  

Effect of Yishen Hugu Decoction on Disused Osteoporosis in Rats

作　　者：高俊[1] 张前德[2] 张曦[1] 胡继红[3] 

机构地区：[1]常州市中医医院骨二科,江苏常州213003 [2]南京医科大学第一临床医学院,南京210029 [3]常州市第二人民医院内分泌研究室,江苏常州213000

出　　处：《中国实验方剂学杂志》2013年第23期179-182,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：高等学校博士学科点专项科研基金(20093237110005);江苏省中医药局科技项目(LZ11207);常州市科技计划项目(CJ20112014)

摘　　要：目的:观察益肾护骨方对废用性骨质疏松大鼠的血尿生化水平、骨结构及股骨生物力学性能影响,探讨益肾护骨方对废用性骨质疏松的改善作用。方法:选取3月龄SD大鼠70只,随机分为正常组、假手术组、模型组、益肾护骨方高、中、低剂量组和阳性药物对照组(强骨胶囊),每组10只。模型组、益肾护骨方高、中、低剂量组和阳性药物对照组行右侧坐骨神经切断术,假手术组的手术过程只分离显露坐骨神经后缝合切口。益肾护骨方高、中、低剂量组分别于造模后第2天予以益肾护骨方52,26,16.25 g·kg-1,阳性药物对照组造模后第2天予以强骨胶囊0.25 g·kg-1ig,用药4周。检测血尿生化、股骨生物力学、胫骨组织形态计量学指标。结果:血碱性磷酸酶(ALP)、血清骨钙素(BGP)、血抗酒石酸酸性磷酸酶(TRACP)、尿羟脯氨酸(HYP)/肌酐(Cr)、骨小梁节点数、游离末端数及股骨生物力学指标,模型组与假手术组相比显著升高或降低(P<0.01);与模型组比较,益肾护骨方高、中、低剂量组血ALP,BGP,TRACP,尿HYP/Cr,骨小梁节点数、游离末端数及股骨生物力学指标均显著降低或升高(P<0.01)。结论:益肾护骨方对废用性骨质疏松大鼠有不同程度的成骨作用,可以不同程度延缓或减轻大鼠废用性骨质疏松的发生。Objective： To study the effect of Yishen Hugu decoction on the changes of the blood and urine biochemical levels,the bone structure,bone biomechanical properties in the rat disused osteoporosis. Method： Seventy SD rats were randomly divided into 7 groups：normal group,sham operation group,model group,high dose,medium dose and low dose of Yishen Hugu decoction group （52,26,16.25 gkg-1）and positive control group （Drynaria fortunei 0.25 g kg-1） with 10 rats in each group.The rats in the model group,high,medium,low dose group of Yishen Hugu decoction and positive control group were performed sciatic neurotomy.Normal group was given regular food and water other groups were gavaged Yishen Hugu decoction respectively,and the rats in positive control group were gavaged Drynaria fortunei. The blood and urine biochemical levels were measured,the femora biomechanical test and the tibia were decalcified and then stained with HE and Masson stain. Result： The differences of alkaline phosphatase（ALP）,osteocalcin（BGP）,tartrate-resistant acid phosphatase（TRACP）,hydroxyproline （HYP）/creatinine（Cr）,bone mineral density（BMD） and the numbers of nodes and free ends in model group and sham operation group were significant statically （P〈0.01）. After administration of Yishen Hugu decoction,those indexes in high,medium,low dose group of Yishen Hugu decoction were obviously higher or lower than that in model group （P〈0.01）. Conclusion： The disused osteoporosis can be prevented effectively by the Yishen Hugu decoction.

关 键 词：废用性骨质疏松 动物模型 骨代谢 生物力学 益肾护骨方 

分 类 号：R285.5[医药卫生—中药学]