新疆阿魏树脂不同分离部位对结肠癌细胞HCT116的抑制作用  

Inhibition Activity of Different Separation Parts of Ferula sinkiangensis Resin on HCT116 Human Colon Cancer Cell

作　　者：周龙龙[1] 张海英[1,2] 任燕[1] 周倩[1] 

机构地区：[1]新疆医科大学,乌鲁木齐830000 [2]新疆医科大学第四附属医院,乌鲁木齐830000

出　　处：《中国实验方剂学杂志》2013年第23期183-186,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金(81260625)

摘　　要：目的:分析新疆阿魏树脂不同分离部位对结肠癌细胞HCT116抑制作用的活性,并确定其有效活性部位。方法:通过磺酰罗丹明B比色法(SRB)与流式细胞术以细胞密度1×105个mL,药物质量浓度250,125,62.5,31.25,15.6 mg·L-1(流式细胞术药物浓度为62.5 mg·L-1),检测新疆阿魏树脂不同分离部位(石油醚部位、乙酸乙酯部位,甲醇部位)对结肠癌细胞HCT116药物作用24 h后的增殖抑制与凋亡作用,以IC50和总凋亡率作为指标衡量其各分离部位抑制肿瘤的活性效能。结果:石油醚部位:对结肠癌细胞HCT116增殖抑制IC5068.7 mg·L-1,总凋亡率为(43.4±1.1)%;乙酸乙酯部位:IC5043.7 mg·L-1,总凋亡率为(56.2±0.9)%;甲醇部位:IC5059.6 mg·L-1,总凋亡率为(46.7±3.1)%。结论:新疆阿魏树脂乙醇提取物的乙酸乙酯萃取部位对结肠癌细胞HCT116细胞毒性作用较强并能促进其大量凋亡,初步确定为新疆阿魏树脂抗肿瘤活性部位。Objective： To study inhibitory activity the different separation parts of Ferula sinkiangensis resin on HCT116 human colon cancer cell and determine its effective active site. Method： The effects of the different separate parts of F. sinkiangensis resin （petroleum ether parts,ethyl acetate parts,methanol parts） on colon cancer cells HCT116 proliferation and apoptosis were observed, IC50 and total apoptosis rate were used toevaluate tumor suppression activity performance by SRB and flow cytometry with cell concentration 1105/mL, and drug concentration of 250,125,62.5,31.25,15.6 mg L-1 （flow cytometry drug concentration was 62.5 mg L-1）. Result： IC50 of petroleum ether unit was 68.7 mg L-1,the total apoptosis rate was （43.41.1）%;IC50 of ethyl parts：43.7 mg L-1,total apoptosis rate was （56.20.9）%;IC50methanol Department of 59.6 mg L-1,total apoptosis rate was （46.73.1）%. Conclusion： Ferula resin ethanol extract ethyl acetate fraction can promote their apoptosis,initially identified as anti-tumor activity in F.sinkiangensis resin parts.

关 键 词：新疆阿魏 结肠癌HCTT116细胞 磺酰罗丹明B比色法 细胞凋亡 抗肿瘤活性 

分 类 号：R285.5[医药卫生—中药学]