RP-HPLC检测大鼠大血管中异钩藤碱的含量  

Detection Content of Isorhynchophylline in Rat Blood Vessels by RP-HPLC

作　　者：杨娟[1] 王兴[1] 李晓倩[1] 陈志远[1] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031

出　　处：《中国实验方剂学杂志》2013年第23期235-238,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30801547);中央高校基本科研业务费专项资金项目(SWJTU11CX115;SWJTU11ZT26)

摘　　要：目的： 建立测定大鼠大血管中异钩藤碱含量的方法。 方法： Wista大鼠灌胃钩藤水煎液后取出大血管，液氮研磨，用乙醇沉淀组织匀浆中的蛋白质，取上清液用氮气吹干，加甲醇复溶后取上清液直接进样，用高效液相色谱仪测定。RP18 WatersTM 色谱柱（4.6 mm250 mm，5 μm），流动相甲醇-水（70∶30），三乙胺调pH 8.0，流速 0.8 mLmin-1，检测波长254 nm，异钩藤碱保留时间约为12 min，柱温 30℃。 结果： 大血管中异钩藤碱的质量浓度在0.04~20.0 mgL-1，大鼠峰面积之比Y与异钩藤碱的质量浓度X具有较好的线性关系，回归方程为Y=4.644 1X＋0.161 2（R2=0.999 2）;平均提取回收率为99.34%，RSD〈8.0%。 结论： 方法简便、稳定、易行，可以用于测定大鼠大血管中异钩藤碱的含量。Objective： To establish a method for the determination of isorhynchophylline on rat great vessels. Method： The rat great vessels were removed after intragastric administration of Uncariae Ramulus Cum Uncis decoction and liquid nitrogen grinding. The tissue samples which were drawn and treated by adding ethanol and the supernatant were dried by N2.After redissolving in carbinol,the supernatant were detected by HPLC directly. The analysis was carried on an analytical column RP18 WatersTM（4.6 mm250 mm,5 μm）. The mobile phase consisted of carbinol-water （70：30）,triethylamine to adjust pH 8.0 and the flow rate was 0.8 mL min-1. The UV detection wave length was at 254 nm and the column temperature was kept at 30℃. The retention time of isorhynchophylline was about 12 min. Result： A good linearity was obtained from 0.04 mg L-1 to 20.0 mg L-1 for isorhynchophylline. Regression curve was Y=4.644 1X＋0.161 2 with a correlation coefficient （R2） of 0.999 2,the average recovery was 99.34%,and the RSD was less than 8.0%. Conclusion： The method is simple,stable and easy for operation. It is shown to be suitable for determination of isorhynchophylline in rat great vessels.

关 键 词：钩藤 异钩藤碱 高效液相色谱 大鼠 大血管 

分 类 号：R284.1[医药卫生—中药学]