淫羊藿-女贞子对GIOP大鼠骨密度和激素水平的相关性分析  

Analysis of Correlation of Combination of Epimedii Folium and Ligustri Lucidi Fructus on Bone Mineral Density and Hormone Levels in Glucocorticoid-induced Osteoporosis Rats

作　　者：康学[1] 周琦[2] 李崭[1] 张伟华[3] 刘仁慧[1] 王秀娟[1] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]首都医科大学附属北京中医医院,北京100010 [3]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《中国实验方剂学杂志》2013年第23期250-253,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：首都中医药及护理学研究专项课题(12ZYH07);北京市教育委员会科技计划面上项目(KM20131002501);北京中医药薪火传承"3+3"工程二室一站建设项目(2012-SZ-C-42)

摘　　要：目的:研究糖皮质激素性骨质疏松(GIOP)大鼠内源性激素水平与骨密度的相关性,探讨淫羊藿-女贞子配伍抗GIOP的作用机制。方法:SD大鼠60只,雌雄各半,随机分为正常对照组、模型对照组、女贞子组、淫羊藿组、淫羊藿-女贞子组、钙尔奇组。地塞米松1 mg·kg-1,im,2次/周,建立骨质疏松模型。造模同时各给药组灌服相应药物,女贞子组灌服女贞子水煎液9.5 g·kg-1,淫羊藿组灌服淫羊藿水煎液9.5 g·kg-1,淫羊藿-女贞子组灌服淫羊藿-女贞子(4∶3)水煎液9.5 g·kg-1,钙尔奇组灌服钙尔奇碳酸钙D3片0.277 3 g·kg-1。8周后处死取材,放射免疫法检测血清皮质醇(COR)、睾酮(T)、雌二醇(E2)含量;双能X射线骨密度仪测量离体股骨骨密度(BMD);并对实验数据进行相关性分析。结果:模型对照组大鼠血清COR,T,E2含量以及股骨不同部位BMD均显著降低(P<0.05或0.01);与模型对照组比较,各给药组均可显著上调血清COR水平(P<0.05或0.01),淫羊藿-女贞子组血清T含量显著升高(P<0.05),女贞子组、淫羊藿-女贞子组血清E2含量显著升高(P<0.05或0.01),且各用药组不同部位BMD均较模型对照组显著升高(P<0.05或0.01);相关性分析结果显示BMD与血清COR,T,E2水平呈显著正相关(P<0.05或0.01)。结论:GIOP大鼠BMD与内源性激素水平密切相关,淫羊藿-女贞子配伍对激素水平的影响可能是其防治GIOP的机制之一。Objective： To explore the correlations between bone mineral density（BMD） and endogenous hormone levels,and then investigate the mechanism of anti-GIOP of combination of Epimedii Folium and Ligustri Lucidi Fructus. Method： Sixty SD rats were randomly divided into 6 groups：normal control group,model group,Epimedii Folium group,Ligustri Lucidi Fructus group,Epimedii Folium and Ligustri Lucidi Fructus mixture group（compatibity group） and positive drug group. All rats received intramuscular injection of dexamethasone （1 mg kg-1,twice a week） for 8 weeks to establish osteoporosis model except those in the control group,rats in treatment groups were treated with intragastric administration of traditional Chinese herbs or positive drug respectively,Ligustri Lucidi Fructus group was given Ligustrum lucidum with 9.5 g kg-1,Epimedii Folium group was given Epimedium with 9.5 g·kg-1, the compatibility group were given Epimedii Folium-Ligust rilucidi Fructus with 9.5 g kg-1 the positive group was administered Caltrate calcium D3 tablets with 0.277 3 g·kg-1. Eight weeks later, serum corticosteroid （COR）,testosterone （T） and estradiol （E2） were determined by radioimmunoassay,and BMD of femur was measured by dual-energy X-ray absorptiometry. Result： The content of serum COR,T,E2 and BMD of modle group were lower than normal control group（P〈0.05 or P〈0.01）. Compared with model group,serum levels of COR were upregulated significantly in each administration group（P〈0.05 or P〈0.01）,serum T levels in compatibility group and E2 levels in Ligustri Lucidi Fructus group and compatibility group were increased significantly（P〈0.05 or P〈0.01）,BMD increased obviously in each treatment group（P〈0.05 or P〈0.01）. The correlation analysis showed that BMD was positively correlated with serum hormone levels（P〈0.05 or P〈0.01）. Conclusion： BMD was closely related to endogenous hormone levels in glucocorticoid-induced osteoporosis rats,which indicate that the effect of combinat

关 键 词：骨质疏松 糖皮质激素 淫羊藿-女贞子 骨密度 内源性激素 相关性 

分 类 号：R285.5[医药卫生—中药学]