HO-1修饰的大鼠骨髓间充质干细胞在体外对淋巴细胞的影响  

Effects of HO-1 modified rat bon e marrow mesenchymal stem cells on lymphocytes in vitro

作　　者：付楠楠[1] 宋红丽[2] 吴本娟 刘涛[4] 

机构地区：[1]天津医科大学一中心临床学院,300192 [2]天津市第一中心医院 [3]天津市器官移植研究所 [4]卫生部危重病急救医学重点实验室

出　　处：《中华微生物学和免疫学杂志》2013年第11期801-806,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金面上项目（81270528）;天津市应用基础与前沿技术研究计划项目（08JCYBJ0）8400,12JCZDJC25200,11JCZDJC27800）;天津市卫生局科技基金面上项目（2011KY11）

摘　　要：目的：探讨血红素加氧酶1（HO-1）修饰的大鼠骨髓间充质干细胞（BM MSCs）在体外对淋巴细胞的免疫调节作用。方法体外分离培养和鉴定BM MSCs ，用载有HO-1基因的重组腺病毒载体进行感染，形成HO-1/BM MSCs，与大鼠脾淋巴细胞共培养。 MTT法检测淋巴细胞的增殖活性，碘化丙啶染色流式细胞术分析细胞周期的分布情况，ELISA法检测细胞因子含量，流式细胞仪分析T淋巴细胞CD25、CD71的表达。结果 HO-1/BM MSCs参与共培养的淋巴细胞的增殖活性和细胞周期进程均被抑制、促炎因子（ IL-2、TNF-α和IFN-γ）的分泌量降低、抗炎因子（ IL-10）的分泌量升高、T淋巴细胞CD25和CD71的表达降低，与BM MSCs组相比差异有统计学意义。结论 HO-1/BM MSCs较BM MSCs能够更好地发挥对淋巴细胞的免疫调节作用。Objective To investigate the immunomodulatory effects of heme oxygenase-1 （ HO-1） modified rat bone marrow mesenchymal stem cells （ BM-MSCs） on lymphocytes .Methods Rat BM-MSCs were cultured and identified in vitro.HO-1 gene was transduced into BM-MSCs via recombinant adenovirus （rAdV） to construct HO-1/BM MSCs, which were then co-cultured with rat spleen lymphocytes .The cellu-lar activity of lymphocytes was detected by using MTT method .The cell cycle distributions were analyzed by propidium iodide staining and flow cytometry .ELISA was used to detect the level of cytokines in the super-natant of cultured cells .The expression of lymphocyte activation markers , CD25 and CD71, were measured by using fluorescence labeled antibodies and flow cytometry .Results Compared with the control group , the lymphocytes co-cultured with HO-1/BM MSCs showed a suppressed proliferation and cell cycle progression . HO-1/BM MSCs inhibited the secretion of IL-2, TNF-αand IFN-γ, but enhanced the secretion of IL-10 in lymphocytes.Furthermore, the expression of CD25 and CD71 were also down-regulated by HO-1/BM MSCs. Conclusion Compared with BM MSCs , HO-1/BM MSCs show higher immunosuppressive effects on lym-phocytes .

关 键 词：骨髓间充质干细胞 血红素加氧酶1 淋巴细胞 免疫调节 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]