血液分离肠球菌毒力基因检测及生物膜形成测定  

Detection of virulence genes and biofilm formation of Enterococci strains isolated from blood samples

作　　者：李东冬[1] 沈定霞[1] 郭玲[1] 马艳宁[1] 

机构地区：[1]中国人民解放军总医院微生物科,北京100853

出　　处：《中华微生物学和免疫学杂志》2013年第11期865-867,共3页Chinese Journal of Microbiology and Immunology

基　　金：首都科学发展基金（2009-1023）

摘　　要：目的：检测肠球菌的主要毒力基因，并测定其生物膜形成情况。方法收集血液来源标本中的粪肠球菌28株，屎肠球菌54株，采用多重PCR检测肠球菌的5种主要毒力基因：asa1、esp、hyl、cylA及gelE；利用微孔板法检测生物膜的形成。结果粪肠球菌中asa1、esp、cylA、gelE的同时检出率为50％，且28株菌均检测到了至少一种毒力基因，仅在粪肠球菌中检测到了asa1、cylA和gelE基因；屎肠球菌对esp的检出率分别为50％，hyl和esp的同时检出率为22．2％，hyl基因仅在屎肠球菌中存在，18．5％的屎肠球菌没有检测到5种毒力基因中的任何一种。粪肠球菌和屎肠球菌形成生物膜阳性菌株的比率分别为85．7％和63．0％。结论致血液感染粪肠球菌在主要毒力基因的种类、毒力基因检出率及生物膜形成阳性菌株数均高于屎肠球菌。Objective To detect the main virulence genes and biofilm formation of Enterococci strains isolated from blood samples .Methods Twenty-eight strains of Enterococcus faecalis （ E.faecalis） and 54 strains of Enterococcus faecium （ E.faecium） were collected from blood samples .Five main virulence genes （asa1, esp, hyl, cylA and gelE） were detected by multiplex PCR.Biofilm formation was investigated by using microtiter dish biofilm formation assay .Results All E.faecalis strains were positive for at least one kind of virulence genes , of which 14 strains were concurrently positive for asa1, esp, cylA and gelE.asa1, cylA and gelE were only detected in E.faecalis strains, while hyl gene only existed in E.faecium strains. Twenty-seven strains of E.faecium were esp positive, of which 12 strains were both hyl and esp positive. None of the 5 virulence genes were identified in 10 strains of E.faecium.85.7% of E.faecalis strains and 63.0%of E.faecium strains could form biofilm.Conclusion Compared with E.faecium strains, more types of virulence genes were detected in E.faecalis strains with higher positive rates .Moreover , E.faecalis strains were more likely to form biofilms than E.faecium strains.

关 键 词：肠球菌 毒力基因 多重PCR 生物膜 

分 类 号：R378[医药卫生—病原生物学]