凡纳滨对虾血蓝蛋白的抗黑曲霉活性  

Anti-Aspergillus niger property of hemocyanin from shrimp Litopenaeus vannamei

作　　者：刘瑶[1] 陈若泓 王泽焕 陈洁辉[1] 张楠[1] 章跃陵[1] 

机构地区：[1]汕头大学理学院生物学系海洋生物研究所,广东汕头515063

出　　处：《中国水产科学》2013年第4期802-807,共6页Journal of Fishery Sciences of China

基　　金：国家自然科学基金项目(30871939;31072237);教育部新世纪优秀人才支持计划项目(NCET-11-0922);广东省自然科学基金项目(10251503101000002;9151064001000001);广东省科技计划项目(2009B020309006);广东省高等学校高层次人才项目(2011)

摘　　要：以凡纳滨对虾(Litopenaeus vannamei)为研究对象,运用亲和层析、SDS-PAGE、Western-blotting、抗真菌实验等技术探索对虾血蓝蛋白的抗黑曲菌活性。结果发现,血蓝蛋白在质量浓度为0.1～0.8 mg/mL时对黑曲霉(Aspergillus niger)生长表现出明显的抑制活性。在此基础上,选取0.2 mg/mL血蓝蛋白作用后的黑曲霉进行显微观察分析。光学显微镜观察结果表明,血蓝蛋白能抑制黑曲霉孢子生长。扫描电镜分析显示,在血蓝蛋白作用下,黑曲霉孢子囊萎缩,分生孢子集聚成团,菌丝裂解、扭曲、变短、无分生孢子囊。由此推测,对虾血蓝蛋白具有抗黑曲菌活性,其抗菌机制可能与血蓝蛋白抑制黑曲菌孢子生长和促使病态菌丝产生等有关。本研究对揭示血蓝蛋白的非特异性免疫机理和寻找新的对虾疾病免疫学防治途径等具有重要意义。We evaluated the anti-Aspergillus niger activity of hemocyanin from shrimp Litopenaeus vannamei using affinity chromatography, SDS-PAGE, western-blotting, antifungal assays, microscope and electron microscopic observation. L.vannamei hemocyanin exhibited anti-A, niger activity at concentrations of 0.1-0.8 mg/mL. Furthermore, microscopic observation suggests that 0.2 mg/mL of hemocyanin inhibits the growth of A. niger spores. Electron microscopic analysis of A. niger indicated that the sporangium were atrophied, and the conidiospores were gathered into gouts. In addition, the hyphaes were cracked, contorted, and shortened, and the conidiosporangia were not visible. Taken together, our results suggest that L.vannamei hemocyanin possesses novel immune function （i.e., anti-Aspergillus niger properties） likely related to inhibition of spore and hyphae growth.

关 键 词：凡纳滨对虾 血蓝蛋白 抗黑曲霉活性 

分 类 号：S941[农业科学—水产养殖]