人早孕绒毛细胞滋养层细胞培养方法的改良  

Isolation and culture of first trimester human trophoblast cell

作　　者：左彦珍[1] 张孔雁 李玉红[3] 许倩[4] 毛晓丹[4] 谭宇思[4] 

机构地区：[1]承德医学院药理学教研室,河北承德067000 [2]承德医学院附属医院老年病科,河北承德067000 [3]承德医学院病理学教研室,河北承德067000 [4]承德医学院基础研究所,河北承德067000

出　　处：《解剖学报》2013年第6期861-864,共4页Acta Anatomica Sinica

基　　金：河北省自然科学基金资助项目(H2012406010);河北省高等学校自然科学研究青年基金资助项目(2010103);承德医学院自然科学基金资助项目(201206)

摘　　要：目的探索一种简便高纯度的早孕滋养细胞的体外培养方法。方法采用完整早孕绒毛通过胰蛋白酶联合胶原酶消化法分离,Percoll梯度分离法提纯体外培养,应用光镜HE染色和免疫细胞化学法检测细胞纯度。结果 CK-7表达阳性细胞数可以达到90%以上。结论完整绒毛消化和Percoll纯化联合可在短期内获得高纯度、高产量的滋养细胞以供后期试验,方法更加简便。Objective To research a simple method for purification of first trimester human trophoblast cell in vitro. Methods Whole first trimester human chorionic tissues were isolated with trypsin/collagenase. HE staining and immunocytoehemistry methods were used to detect the tyophoblast cells purity. Results Cytokeratin-7 positive cells were more than 90%. Conclusion First trimester human Trophoblast cells can be obtained through the simple method and may offer enough cells for later experiments.

关 键 词：滋养细胞 早孕 原代细胞培养 Percoll分离 人 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]