降钙素对骨质疏松性骨折修复过程胶原基因表达的影响  

Effect of calcitonin on the expression of collagen gene during the healing process of osteoporotic fractures

作　　者：肖强[1] 刘静莉[2] 漆启华[1] 陆欢[1] 邓亮[1] 董谢平[1] 

机构地区：[1]江西省人民医院骨科,江西南昌330006 [2]南昌市洪都中医院骨科,江西南昌330008

出　　处：《中国骨质疏松杂志》2013年第11期1170-1173,共4页Chinese Journal of Osteoporosis

摘　　要：目的探索降钙素对骨质疏松性骨折愈合过程中基质Ⅰ、Ⅱ、Ⅲ型胶原mRNA表达的影响,阐明降钙素促进骨质疏松性骨折愈合的机制。方法选用96只2个月龄SD雌性大鼠,64只行双侧卵巢切除(OVX)后,随机平分为降钙素组和对照组,其余32只为假手术组;10周后所有大鼠在右侧桡骨下端制作1 mm骨缺损的骨折愈合模型。降钙素组从骨折术前1 w至术后8 w连续每天皮下注射鲑鱼降钙素2 IU/kg,另2组注射等量生理盐水。采用原位杂交技术分析7 d,14 d,28 d,56 d,4个时间、点降钙素对骨折修复过程中基质Ⅰ、Ⅱ、Ⅲ型胶原mRNA表达的影响。结果降钙素治疗下,骨基质Ⅰ型胶原mRNA表达的量与对照组比较有显著性差异,假手术组>降钙素组>对照组;Ⅲ型胶原mRNA的表达变化则与之相反。降钙素组软骨性骨痂向骨性骨痂转换时间较对照组缩短。结论降钙素能影响胶原mRNA的表达,明显促进骨质疏松性骨折的愈合。Objective To investigate the effect of calcitonin on the mRNA expression of matrix type I, Ii, and III collagen during the healing process of osteoporotic fractures, and to illuminate the mechanism of calcitonin in promoting the healing of osteoporotie fractures. Methods A total of 96 2-month female SD rats were selected. Of which, 64 rats received bilateral ovariectomy （OVX） , and they were randomly divided into calcitonin therapy group and control group. The other 32 rats were divided into sham operation group. Ten weeks later, all the rats made into fracture models with about lmm width of the bone defect at the end of the right radius. Rats in the calcitonin therapy group received a subcutaneous injection of 2 IU/kg salmon ealcitonin once a day from 1 week before the operation to 8 weeks after the operation. Rats in the other 2 groups were injected with same volume of normal saline. The effect of ealcitonin on the mRNA expression of type I, II, and III collagen during the healing process of bone fractures was observed using in situ hybridization on the 7th, 14th, 28th, and 56th day. Results With the treatment of caleitonin, the expression of matrix type I collagen mRNA was significantly different to that in control groups, which indicated as sham operation group 〉 calcitonin therapy group 〉 control group. Whereas, the expression of type III collagen mRNA was opposite to that of type I collagen mRNA. The period in calcitonin therapy group transferring from cartilaginous callus into bony callus was shorter than that in control group. Conclusion Calcitonin can affect the expression of collagen RNA and promote the healing process of osteoporotie fractures obviously.

关 键 词：骨质疏松 骨折愈合 胶原基因 降钙素 

分 类 号：R68[医药卫生—骨科学]