浙江南部地区416例肾移植受者KIR基因多态性分析  

Study on KIR gene polymorphisms in 416 renal transplantation recipients from southern Zhejiang

作　　者：陈孝倩[1] 吴琦[3] 谢双双[2] 赵卫军 吴存造[2] 蔡勇[2] 夏鹏[1] 杨亦荣[1] 陈必成[2] 

机构地区：[1]浙江省温州医科大学附属第一医院肾病实验室,325000 [2]浙江省温州医科大学器官移植中心,325000 [3]浙江省丽水市人民医院泌尿外科 [4]天津秀鹏生物有限公司

出　　处：《中华医学遗传学杂志》2013年第6期701-705,共5页Chinese Journal of Medical Genetics

基　　金：温州市科技计划资助项目（H20100070）,浙江省自然科学基金（Y2110944,LY12H1004）

摘　　要：目的分析浙江南部地区同种异体肾移植受者杀伤细胞免疫球蛋白样受体（ki11erce11immunog1obu1in-1ikereceptor，K／R）基因多态性的分布情况。方法采用序列特异性引物聚合酶链反应检测浙江南部地区无血缘关系的416例汉族肾移植受者的KIR基因型，并与其他地区人群的基因型相比较。结果共检出16个已知的KIR基因，其中2DL4、3DL2～3、3DPI在所有个体中均表达；沉默基因2DPI的基因型频率为99％。其它高频的基因依次为2DLI、2DL3、3DLI、2DS4（92．1％～98．8％）。抑制型基因2DL2基因表型频率（34．6％）高于江浙沪汉族人群（P〈0．05）；比较其他基因表型频率差异无统计学意义（P〉0．05）。检出的41种KIR基因型中有3种基因型未见文献报道。KIR基因型以AA1型频率最高（43．51％），明显低于已报道的浙江北部和江苏地区对照人群。结论浙南地区肾移植受者K／R基因分布与华东地区健康对照人群基本一致，但也表现出其独特的K／R基因多态性特征。Objective To investigate polymorphisms of killer cell immunoglobulin-like receptor gene （KIR） in renal transplant recipients from southern Zhejiang. Methods KIR genotypes were analyzed by PCR-SSP in 416 renal transplant recipients, and the genotype frequencies were compared with populations from Eastern China and worldwide. Results All 16 known KIR genes were detected in the renal transplant recipients, and KIR2DL4, 3DL2-3, 3PD1 were found in all. As a pseudogene, 2DP1 has a high genotype frequency （99%）. The frequencies of KIRgDL1, 2DL3, 3DL1, 2DS4 have ranged from 92.1% to 98.8%. Compared with 11 groups in Eastern China and other countries, the KIR2DL2 phenotype frequency was higher （34.6~） than those of Shanghai, Zhejiang and Jiangsu populations （P〈 0.05）. Among 41 genotypes, three have not been reported previously. The most common genotype was AA1, with a frequency of 43.51%, which was significantly lower than those of Jiangsu and Northern Zhejiang. Conclusion Renal transplant recipients from southern Zhejiang share similar features with Eastern China Han population with regard to KIR polymorphisms, but also have unique frequencies for KIR genotypes.

关 键 词：杀伤细胞免疫球蛋白样受体 肾移植 基因型 多态性 

分 类 号：R699[医药卫生—泌尿科学]