小麦品系渝356-9抗叶锈基因的初步研究  

Study of Leaf Rust Resistance Gene in Wheat Line Yu 356-9

作　　者：韩柳莎[1] 康占海[1] 王佳真[1] 李星[1] 刘大群[1] 

机构地区：[1]河北省农作物病虫害生物防治工程技术研究中心,河北农业大学保定071001

出　　处：《分子植物育种》2013年第6期741-745,共5页Molecular Plant Breeding

基　　金：中国博士后基金项目(2011M500672);河北省教育厅优秀青年基金项目(YQ2013024)共同资助

摘　　要：中国小麦品系渝356-9在苗期和成株期都表现良好的抗叶锈性,本研究利用小麦叶锈菌生理小种FHNQ接种渝356-9、郑州5389杂交获得的F2群体,分别进行温室和田间抗叶锈性鉴定,鉴定结果经卡方检验,均符合3:1的理论分离比例,表明亲本渝356-9的抗性是由一对显性基因控制,暂命名为LrYu。利用分离群体分组分析法和分子标记技术对F2群体进行分子标记检测,运用MapManager QTXb20软件对LrYu进行连锁分析并构建遗传图谱。标记分析表明LrYu与2BS上的3个SSR标记紧密连锁,其中距离最近的标记是Xgwm410,与LrYu遗传距离是9.3 cM。Chinese wheat line Yu 356-9 has a highly effective seedling and adult leaf rust resistance. A F2 popul- ation from the cross Yu 356-9/Zhengzhou 5389 was inoculated by Puccinia triticina pathotype FHNQ in the greenhouse and field, respectively. The ratios of F2 population segregation fit 3：1, showing that resistance of Yu 356-9 was conferred by a dominant gene, tentatively designated LrYu. Bulked Segregate Analysis and molecular markers were used to map LrYu. Linkage analysis was conducted using the software MapManager QTXb20. Results indicated that three SSR markers on 2BS were closely linked to LrYu. Xgwm410 with genetic distance of 9.3 cM is the closest one.

关 键 词：小麦叶锈病 抗病基因 SSR 分子作图 

分 类 号：S512.1[农业科学—作物学]