转AtAMT1;1基因甜菜T_1代基因表达及其功能鉴定  

The Molecular Detection and Functions Identification of T_1 Generation of AtAMT1;1 Transgenic Sugar Beets

作　　者：张少英[1] 段娜[1] 孙亚卿[1] 于超[1] 张永丰[1] 

机构地区：[1]内蒙古农业大学甜菜生理研究所,呼和浩特010018

出　　处：《分子植物育种》2013年第6期762-766,共5页Molecular Plant Breeding

基　　金：现代农业产业技术体系建设专项资金(CARS-210304)资助

摘　　要：甜菜是中国重要的糖料作物,为提高甜菜氮素利用效率,对其进行了高亲和铵转运蛋白基因的遗传转化。本研究主要对转AtAMT1;1基因甜菜T1代植株进行了PCR、RT-PCR检测和相关生理特性的分析。结果表明,PCR和RT-PCR检测的T1代转基因甜菜呈阳性,并在根中表达;在控制氮素供应的条件下,转基因植株的叶绿素和氨态氮含量显著高于野生型甜菜,块根产量和含糖率也高于对照,初步证明转AtAMT1;1基因甜菜可提高氮素的利用效率。Sugar beet is an important sugar crop, to improve beet nitrogen use efficiency, its genetic transform- ation of the high-affinity ammonium transporter gene was done. This experiment takes a molecule detection and physiological characteristics analysis to the T1 generation ofA tA MT1;1 transgenic sugar beet. The results show that the PCR detection and RT-PCR detection of T~ generation of transgenic sugar beet is positive. In the control of nitrogen supply conditions, transgenic plants chlorophyll content and ammonia nitrogen content enhance than the control of wild-type sugar beet, and the difference was significant. Transgenic sugar beet yield and sugar content higher than the wild type, preliminary evidence AtAMT1;1 transgenic sugar beet can increase the efficiency of nitrogen utilization.

关 键 词：甜菜 AtAMT1 1基因 转基因 氮素利用 

分 类 号：S566.3[农业科学—作物学]