钩吻素子抗外周神经损伤致神经病理性疼痛中自发痛的效应  

Therapeutic Effects of Koumine on Spontaneous Pain of Peripheral Nerve Injury-induced Neuropathic Pain

作　　者：李苏平[1] 许盈[1] 凌倩[1] 洪志红 刘铭[1] 杨渐[1] 俞昌喜[1] 

机构地区：[1]福建医科大学药学院药理学系,福州350004

出　　处：《福建医科大学学报》2013年第4期204-209,共6页Journal of Fujian Medical University

基　　金：国家自然科学基金面上项目(81273493);国家青年科学基金项目(81200868);福建省自然基金项目(2009J01156);福建省改革与发展委员会产业技术开发项目(闽发改投资[2009]958号);福建医科大学重大科研项目(09ZD009)

摘　　要：目的观察钩吻素子抗外周神经损伤致神经病理性疼痛(NPP)中自发痛的效应。方法在大鼠坐骨神经慢性束缚损伤(CCI)NPP模型上,以术侧自发性抬足时间和足姿势评分为指标,观察单次(7.0,1.4,0.28mg/kg)、连续多次(7.0,0.28mg/kg,每日2次,连续7d)皮下注射给予钩吻素子对NPP中自发痛的效应。结果单次皮下注射给予钩吻素子剂量依赖性减少自发性抬足时间,其中7.0mg/kg组与对照组比较差别有统计学意义(P<0.01);单次皮下注射给予钩吻素子剂量依赖性降低足姿势评分,其中1.4,7.0mg/kg组与对照组比较差别有统计学意义(P<0.05和P<0.01)。连续多次给予钩吻素子减少自发性抬足时间,7.0mg/kg组在术后第3,5,7,9d自发性抬足时间均低于对照组(P<0.01);连续多次给予钩吻素子降低足姿势评分,7.0mg/kg组在术后第3,5,7,9d评分均低于对照组(P<0.05或P<0.01),到术后第9d疗效最强。结论钩吻素子具有抗外周神经损伤致NPP中自发痛的效应。Objective To investigate the nerve injury-induced neuropathic pain. Methods effects of Koumine on spontaneous pain of peripheral Rat chronic constriction injury （CCI） model of neuro- pathic pain was used to observe the effects of Koumine administered subcutaneously one time at dose of 7.0, 1.4 or 0.28 mg/kg, or twice a day for 7 consecutive days at 7.0 or 0.28 mg/kg on spontaneous pain indicated by ipsilateral foot-lifting time and postural index. Results Single dose of Koumine reduced both foot-lifting time and the postural index in a dose-dependent manner, in particular, the difference in the foot-lifting time between 7. 0 mg/kg group and untreated control group was statistically significant （P〈0.01） whereas the significant difference in the postural index was observed in both 1.4 and 7.0 mg/kg group as compared with the control group （P〈0.05 and P〈0.01 respectively）. Repeated administration of 7.0 mg/kg Koumine shortened spontaneous foot-lifting time at days 3, 5, 7 and 9 post CCI （P〈0.01）. Similarly, Koumine repeated dosing at 7.0 mg/kg also ameliorated the postural index at days 3, 5, 7 and 9 after the injury （P〈0.05 or P〈0.01） with the therapeutic effect being most prominent at day 9 after the CCI operation. Conclusion Koumine has pain-relieving effect on spontaneous pain of peripheral nerve injury-induced neuropathic pain.

关 键 词：钩吻 钩吻碱 钩吻素子 镇痛 周围神经 疼痛 创伤和损伤 神经痛 自发痛 

分 类 号：R285.5[医药卫生—中药学]