白藜芦醇对急性痛风性关节炎大鼠趋化因子CCR5、CXCL10的影响  

The effect of resveratrol on chemokines CCR5, CXCL10 in rats with acute gouty arthritis

作　　者：王鹏[1] 丁慧[1] 陈颖[1] 王忠超[1] 孙晓方[1] 王颜刚[1] 

机构地区：[1]青岛大学医学院附属医院内分泌科,266003

出　　处：《中华内分泌代谢杂志》2013年第11期986-990,共5页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的观察白藜芦醇对急性痛风性关节炎大鼠血清及关节液中趋化因子CCR5、CXCL10的影响。方法选取Wista大鼠36只．按随机数字表法分为空白对照组、模型组、秋水仙碱组、白藜芦醇低剂量组、白藜芦醇中剂量组、白藜芦醇高剂量组，各组相应采用生理盐水、秋水仙碱、白藜芦醇低、中、高剂量灌胃7d（1次／d），模型组及各实验组于灌胃第4天将25mg/ml（0．05ml）浓度的尿酸盐溶液注射到大鼠踝关节腔内，制备急性痛风性关节炎模型，空白对照组大鼠关节腔内注射生理盐水0．05ml，分别于造模后2、9、24、48、72h取血．72h后留取踝关节关节液，观察各组不同时间点关节肿胀指数的变化及血清关节液中白细胞介素（IL）-1p、CCR5、CXCL10的变化。结果关节液中，低、中、高剂量组IL-1p含量分别为（9．63±0．71）、（7．674-0．48）和（6．664-0．29）pg／ml，与模型组（11．85±0．45）pg／ml比较明显下降（P〈0．05），中、高剂量组CCR5含量分别为（4．49±0．25）和（4．65±0．25）ng／ml，与模型组（5．64±0．28）ng／ml比较明显下降（P〈0．05）＋中高剂量组CXCL10含量分别为（86．79±2．90）和（89．61±5．47）pg／ml，与模型组（110．13±4．02）pg／ml比较明显下降（P〈0．05），病理结果显示，白藜芦醇可减轻急性痛风性关节炎大鼠踝关节组织的水肿和炎性细胞浸润。结论急性痛风性关节炎发病过程中IL-1p、趋化因子CCR5、CXCL10明显增高，白藜芦醇可有效抑制急性痛风性关节炎发作．目．该作用呈-守的剂量依籁件．Objective To study tile effects of resveratrol on chemokines CCR5, CXCL10 of serum and synovial fluid in acute gouty arthritis of rats. Methods According to random number table, 36 Wista rats were divided into six groups ： i. e. control group, model group, colchicine group, high dose resveratrol group （ HR ） , medium dose resveratrol group （ MR）, and low dose resveratrol group （ LR ）. The rats were respectively administrated with normal saline, colchicine suspension, high dose resveratrol, medium dose resveratrol, low dose resveratrol far 7 days, once a day. Rats acute gouty arthritis modles were estalished on the fourth day by monosodium urate solution （concentration of 25 mg/ml, 0.05 ml） injected into the ankle cavity, while 0.05 ml normal saline was injected into joint cavity of rats in control group. 2, 9, 24, 48, 72 hours after model estabifishment, blood samples were collected from inner canthus to detect levels of interleukin （IL） -1 [3, CCR5, CXCL10. 72 hours after model estabilishment, synovial fluid were collected from ankel to detect levels of IL-113, CCRS, CXCL10. The joint swelling index was observed periodically. Results The levels of IL-1 [3 in the low, medium, and high doses of resveratrol were （9.63 ±0.71 ）, （ 7.67 ± 0.48 ）, and （ 6.66 ± 0.29 ） pg/ml respectively, being significantly lower than that in the model group （ 11.85 ± 0.45 ） pg/ml（ P〈0.05 ） in joint fluid. The levels of CCR5 in the medium and high doses of resveratrol groups were（4.49 ± 0.25 ） and （ 4.65 ± 0.25 ） ng/ml respectively, being significantly lower than that in the model group （5.64 ± 0.28 ）ng/ml （P〈0.05）. The levels of CXCL10 in the medium and high doses of resveratrol groups were significantly lower than that in the model group[ （86. 79±2.90）and（g9.61±5.47）vs （110. 13 ±4.02）pg/ml, P〈 0.05 ]. The histopathologic results showed that resveratrol might reduce acute gouty arthritis joint edema, improve inflammatory cells infiltrating. Conclusion

关 键 词：痛风性关节炎 白藜芦醇 趋化因子 

分 类 号：R285.5[医药卫生—中药学]