跨种扩增筛选大鳄龟微卫星标记及遗传多样性分析  

Isolation of Microsatellite Markers by Cross-amplification and Genetic Diversity Analysis in Macroclemys temminckii

作　　者：闵金金[1,2] 张加勇[1] 郑荣泉[1,2] 包得超 

机构地区：[1]浙江师范大学生态研究所,金华321004 [2]浙江师范大学特种水产研究所,金华321004 [3]浙江师范大学化学与生命科学学院,金华321004

出　　处：《动物学杂志》2013年第6期926-932,共7页Chinese Journal of Zoology

基　　金：浙江省"十二五"水产育种重大专项(No.2012C12907-9);浙江省重点科技创新团队自主确定项目(No.2010R50026-07);浙江省大学生科技创新活动计划资助项目(No.2012R404068);金华市科技局重点项目(No.2010-2-012)

摘　　要：跨种扩增是一种能够快速、有效地获得物种微卫星标记的方法。本研究利用在近缘种中已发表的微卫星DNA引物，对大鳄龟（Macroclemys temminckii）进行跨种PCR扩增，在合成的69对引物中获得8对具有多态性的微卫星位点。对PCR扩增产物进行统计，得出观测杂合度（H0）的范围是0．0417～0．9545，平均为0．384 8；期望杂合度（HE）的范围为0．0417—0．8118，平均为0．4791；多态信息含量范围为0．0400～0．7592，平均为0．4232；经过卡方检验后，部分微卫星位点符合哈迪一温伯格平衡。总体来说，这些位点是研究大鳄龟遗传结构的良好分子标记。The method of cross-amplification can be used to obtain microsatellite marker quickly and effectively. This study amplified microsatellite loci of Macroclemys temminckii using published microsatellite DNA primers from related species. In M. temminckii, 8 microsatellite loci which had polymorphism were found in 69 microsatellite markers. By analyzing PCR amplification products, a total of 30 alleles were detected, and the average number of alleles was 3.75. The observed heterozygosity （Ho） ranged from 0. 041 7 to 0. 954 5, and expected heterozygosity （HE） was 0. 041 7 to 0. 811 8. PIC value was from 0. 040 0 to 0. 759 2, averaging 0. 423 2. After chi-square test, some loci were found to correspond to Hardy-Weinberg equilibrium. In general, these loci are good molecular markers that can be used for studying genetic structure for M. temminckii.

关 键 词：大鳄龟 微卫星标记 跨种扩增 遗传多样性 

分 类 号：S917.4[农业科学—水产科学]