脂多糖对哮喘小鼠肥大细胞活化及p38蛋白激酶表达的影响  

Effect of Lipopolysaccharide on Activation of Mast Cell and The Expression of p38 Mitogen-activated Protein Kinase in Asthmatic mice

作　　者：胡克[1] 肖锦秀[1,2] 黄翠萍[2] 

机构地区：[1]武汉大学人民医院呼吸内科,武汉430060 [2]湖北科技学院临床医学院,咸宁437100

出　　处：《中国医药导刊》2013年第10期1695-1696,共2页Chinese Journal of Medicinal Guide

基　　金：湖北省教育厅资助项目(编号:D20092801)

摘　　要：目的:探讨脂多糖(LPS)对哮喘小鼠肥大细胞活化及p38蛋白激酶(p38MAPK)表达的影响。方法:将24只BALB/c小鼠随机分为3组:A(哮喘组)、B(0.1mg LPS+OVA组)、C(对照组),每组8只,采用免疫佐剂(氢氧化铝)与卵清蛋白(OVA)腹腔注射致敏及用1%OVA生理盐水雾化激发的方法制备哮喘小鼠模型。采用免疫组织化学染色SP法半定量测定肺组织中类胰蛋白酶及p38MAPK表达的情况,HE染色观察肺组织病理变化。结果:与A组相比,B组小鼠肺组织类胰蛋白酶及p38MAPK的MOD显著升高(P<0.05);光镜下,A组支气管黏膜下水肿,管壁平滑肌肥大,基底膜增厚,黏膜皱襞增多,气道、血管周围间质可见大量嗜酸性粒细胞、中性粒细胞、淋巴细胞为主的炎症细胞浸润。B组上述情况未减轻,并出现肺泡腔及间质充血。C组气道周围无炎症细胞浸润,肺泡壁结构完整。结论:LPS可明显加重哮喘气道炎症,表明部分机制可能是通过影响肥大细胞活化及p38MAPK信号通路实现的。Objective：To investigate the effects of lipopolysaccharide on activation of mast cell and the expression of p38 mitogen-activated protein kinase （p38MAPK） in asthmatic mice.Methods：Twenty-four BALB/c mice were randomly assigned to three groups,group A（asthma group）、group B（0.1mg LPS＋OVA group）、group C（control group）, with 8 mice in each group. Mice in the group A and group B were treated with adiuvant （aluminum hydroxide） and chicken ovallbumin （OVA）.The expression of tryptase and p38MAPK were semi-quantitative detection by using immunohistochemistry sp method and the pathologic changes of lung tissue were observed by HE stain.Results：The results of immunohistochemical determination showed that lung tissue of mice tryptase and p38MAPK in the group B more than those of the group A（P〈0.05）.The lung tissue from group A showed bronchial submucosal edema,smooth muscle hypertrophy,basementmembra thickening,mucosa plica increasing,inflammatory cells infiltration in the peribronchovascular interstitium.Bisides,the lung tissue of group B was also obviously observed alveolar and infiltration hyperemia.So the pathological changes in the group B were worse than that of the group A.However,the group C was no inflammation cell around airway.Conclusion：The results of LPS exacerbating airway inflammation in asthma may be achieved by influencing by the activation of mast cells and influencing p38MAPK signal pathway.

关 键 词：脂多糖(LPS) 哮喘 p38蛋白激酶(p38MAPK) 类胰蛋白酶 

分 类 号：R562.25[医药卫生—呼吸系统]