Clara细胞分泌蛋白对创伤性休克肺损伤的保护作用  

Protective effects of clara cell secretary protein on lung injury in traumatic shock

作　　者：李菲[1] 宋志[1] 崔岩[1] 金红旭[1] 侯明晓[1] 高燕[1] 

机构地区：[1]解放军沈阳军区总医院急诊科全军重症战创伤救治中心,沈阳110016

出　　处：《临床急诊杂志》2013年第11期522-524,527,共4页Journal of Clinical Emergency

基　　金：全军医学科技"十二五"科研项目(No:CSY12J002)

摘　　要：目的:探讨Clara细胞分泌蛋白(CC16)对创伤性休克大鼠模型继发性肺损伤的保护作用及其机制。方法:选择30只成年雄性SD大鼠,随机分为3组:假手术组、对照组、CC16处理组。采用创伤性休克动物模型,假手术组均完成所有手术操作,但不放血和复苏;对照组放血后在液体复苏前雾化吸入与CC16处理组等容量的生理盐水;CC16处理组在复苏前给予雾化吸入0.1μg/ml的重组人Clara细胞分泌蛋白(rh-CCSP)。实验结束后监测动脉血气分析及肺湿/干重比等指标变化,并测定肺组织中丙二醛(MDA)、髓过氧化物酶(MPO)及肺组织病理学的改变。结果:与对照组相比,CC16处理组的pH、PaO2值明显升高,BE值降低(P<0.05),肺湿/干重比减少(P<0.05);CC16处理组与假手术组间比较差异无统计学意义(P>0.05);与对照组比较CC16处理组的MDA、MP0含量明显降低,肺组织病理学形态也有明显改善。结论:雾化吸入rh-CCSP可减轻创伤性休克大鼠继发性肺损伤的炎症反应,对肺组织具有保护作用。Objective：To investigate the effect and mechanisms of Clara cell secretary protein（CC16） on traumatic shock induced lung injury of rats.Method：Thirty SD male rats were randomly assigned to three groups： sham-surgery group,control group and CC16-treatment group.The animal model of traumatic shock was used in the research.Rats of sham-surgery group were operated,but not treated by depletion and recovery.Rats of control group inhaled after the physiological saline before fluid resuscitation.Rats of CC16-treatment group inhaled 0.1 μg/ml of recombinant human Clara cell secretary protein（rh-CCSP）before fluid resuscitation.Arterial blood gas analysis and wet/dry weight were detected.The lung tissue malondialdehyde（MDA）,myeloperoxidase（MPO）and lung tissue pathology change were observed.Result：Compared with control group,pH and PaO2 value in CC16-treatment group significantly increased.Meanwhile,BE value decreased and lung wet/dry weight ratio decreased（P0.05）.Compared with control group,contents of MDA and MPO in CC16-treatment group obviously decreased.Lung tissue pathology changed obviously.Conclusion：Inhalation of Rh-CCSP could have potential protective effect on lung tissues of rats with traumatic shock.

关 键 词：CLARA细胞分泌蛋白 休克 创伤性 肺损伤 大鼠 

分 类 号：R605.971[医药卫生—急诊医学]