MSM HIV感染者特异性T细胞TCR α CDR3谱序初探  

Detection the TCR CDR3 shewing of TCRαgene repertoire in the MSM HIV infected people

作　　者：李志峰[1] 冯连贵[1] 王豫林[1] 曾艺[2] 张宏军 冷文杰 陈亮 李琴 

机构地区：[1]重庆市疾病预防控制中心 [2]重庆市渝中区疾病预防控制中心 [3]永川区疾病预防控制中心,重庆400042

出　　处：《疾病监测与控制》2013年第12期713-715,共3页Journal of Diseases Monitor and Control

基　　金：重庆市自然科学基金项目(cstc2011jjA10099);重庆市卫生局重点课题(2011-1-094)

摘　　要：目的监测HIV感染者的外周血样本TCRα链CDR3谱系变化,发现HIV特异性TCR TRAV家族。方法用本实验室已建立的"荧光定量PCR溶解曲线法监测HIV感染者TCR CDR3谱系漂移技术",对22名健康男性、19名MSM HIV感染者的外周血样本TCRα链CDR3变化进行监测。结果MSM HIV感染者的TRAV4.2,TARV10和TRAV23的单(寡)性表达频率要高于健康男性人群(P<0.05),MSM HIV感染者的TRAV亚家族的缺失率(1.70%)虽然高于对照男性健康人群的TRAV亚家族的缺失率(0.80%),但二者没有统计学差异。结论在国内首次利用"荧光定量PCR溶解曲线法"监测HIV感染者TCRα链CDR3漂移变化,发现MSM HIV感染者的TCR AV家族存在单(寡)性增生和缺失现象,非正态分布现象增加,多样性减少。Objective To monitor the change of the TCR ct CDR3 spectratyping and find the special T cell receptor （TCR） TRAV family in peripheral bloods of the HIV infections. Methods Monitor the change of the TCR ct CDR3 spectratyping in 19 MSM HIV infections and 22 healthy male populations by using the established method quantitative PCR fluorescence melting curve analysis in our laboratory. Results The TRAV4.2, TARV10 and TRAV23 monoclonal or oligoclonal in MSM HIV infections were higher than in healthy male people （P〈0.05）, and the lack frequency of TRAV sub-family in MSM HIV infections was higher than in healthy male people, but no Significant statistics difference （P〈0.05） . Conclusion The phenomenon of monoclonal（or oligoelonal） proliferated, lacked, abnormal distribution increased and the diversity diminished in the TCR BV family were found in these of MSM HIV infections.

关 键 词：HIV TCR CDR3 谱系漂移 

分 类 号：R512.91[医药卫生—内科学]