高效液相色谱测定预调酒中喹啉黄的方法  

Determination of quinoline yellow in Rum Sparkling by HPLC

作　　者：房宁[1] 巩俐彤[1] 李倩[1] 

机构地区：[1]北京市大兴区疾病预防控制中心,北京102600

出　　处：《中国卫生检验杂志》2013年第15期3049-3050,3053,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立高效液相色谱测定预调酒中喹啉黄的方法。方法样品中喹啉黄经聚酰胺固相萃取柱提取、净化后,用高效液相色谱法测定,以保留时间定性,峰面积定量。结果喹啉黄在0.8 mg/L^20 mg/L范围内线性良好,方法检出限为0.0017 g/L,加标回收率为87%~102%,相对标准偏差(RSD)小于8%。结论本法简便、快速、准确,灵敏度及精密度高,适用于预调酒中喹啉黄的测定。Objective To establish a method for determination of quinoline yellow in Rum Sparkling by HPLC. Methods The quinoline yellow in samples were determined by HPLC after extraction and purification on polyamide solid phase extraction column, with the retention time for qualitative analysis and peak area for quantitative analysis. Results The linear range of quinoline yellow was 0.8 mg/L - 20 mg/L, the quantification limit was 0.0017 g/L, recoveries were 87% - 102% with the relative It has been successfully applied to determination of quinoline yellow in Rum Sparkling.

关 键 词：高效液相色谱法 预调酒 喹啉黄 ProElut PLS固相萃取柱 

分 类 号：O657.72[理学—分析化学]