microRNA-208与微创冠脉搭桥手术后心肌损伤的相关性研究  

Correlation of serum microRNA- 208 and myocardial injury in the patients after OPCABG operation

作　　者：张文静[1] 蔡成松[2] 黄玲玲[2] 李祥云[2] 

机构地区：[1]杭州师范大学附属医院ICU科,浙江杭州310015 [2]杭州师范大学附属医院检验科,浙江杭州310015

出　　处：《中国卫生检验杂志》2013年第16期3207-3209,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省自然科学基金项目(LY12H19001);杭州市医药卫生科技计划项目(20100633B17)

摘　　要：目的探讨血清中microRNA-208(miRNA-208)的表达量与微创冠脉搭桥手术(OPCABG)术后心肌损伤的相关性。方法收集健康对照组和OPCABG组术前患者各30例,分别在术前和术后8 h、12 h、24 h和48 h等5个时间点采集血液,利用实时荧光定量PCR法和酶联荧光分析法对血清中miRNA-208和肌钙蛋白I(cTnI)进行测定,对结果进行统计分析;并对OPCABG组miRNA-208和cTnI在术后4个不同时点的相关性进行分析。结果 miRNA-208在OPCABG组术前患者血清中的表达量与对照组比较差异无统计学意义(P>0.05),与术前相比,术后miRNA-208的表达量升高,术后24 h时达到最高;术后4个不同时点miRNA-208与cTnI均具有较强的相关性(r=0.71,r=0.74,r=0.77,r=0.76;P<0.05)。结论 miRNA-208可能作为反映OPCABG术后心肌损伤的一种新的潜在生物学指标。Objective To explore the correlation between serum microRNA - 208 （ miRNA - 208 ） and myocardial injury in pa- tients after off - pump coronary artery bypass grafting（OPCABG） operation. Methods miRNA - 208 and eTnI levels were de- tected with real time fluorescence quantitative PCR （ FQ - PCR） and enzyme linked immunofluorescent analysis methods in 30 healthy controls and 30 OPCABG patients at five different time points before and after the operation. The results were compared and analyzed with statistical methods and correlation analysis. Results Serum miRNA -208 level was not obviously higher in OPCABG group before operation than that in the healthy controls（ P 〉 0.05 ）. Serum miRNA -208 level gradually increased and reached the peak level at 24 h after operation. There was a strong correlation between miRNA - 208 levels and eTnI levels （r=0.71, r=0.74, r=0.77, r=0.76; P〈0.05） at four different time points after operation. Conclusion Serum miRNA -208 may be a new potential biomarker to reflect myocardial injury of OPCABG.

关 键 词：微创冠脉搭桥手术(OPCABG) microRNA-208(miRNA-208) 肌钙蛋白I(cTnI) 

分 类 号：R542.2[医药卫生—心血管疾病]