柿叶黄酮磷脂复合物的制备工艺研究  

Preparation technology for phospholipids complex of total flavonoids from leaves of Diospyros kaki

作　　者：张胜海[1] 王英姿[1] 段飞鹏[1] 李彩霞[1] 冯艾灵[1] 孟繁蕴[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京师范大学中药资源保护与利用北京市重点实验室,北京100875

出　　处：《中草药》2013年第22期3136-3140,共5页Chinese Traditional and Herbal Drugs

基　　金：北京市科技新星项目(2008A057);北京中医药大学复方中药制药研究创新团队项目资助(2011-CXTD-13);北京中医药大学自主选题在读研究生资助项目"大鼠肠内菌对柿叶黄酮多成分代谢转化的研究"

摘　　要：目的考察柿叶黄酮磷脂复合物制备过程中的影响因素,并确定最佳制备工艺。方法采用溶剂法制备柿叶黄酮磷脂复合物,以柿叶黄酮中黄酮类化合物与磷脂的复合率为评价指标,结合单因素考察和正交试验,确定其最佳制备工艺条件;采用X-射线衍射、差示扫描量热分析(DSC)对复合物的形成进行验证。结果反应溶剂、柿叶黄酮与大豆磷脂投料比是磷脂复合物制备过程中的主要影响因素;最佳制备工艺条件为无水乙醇作为反应溶剂,柿叶黄酮与大豆磷脂投料比1∶2,反应物质量浓度30 mg/mL,在30℃下反应1 h;在此条件下复合率为96.95%。结论本研究建立的柿叶黄酮磷脂复合物最佳制备工艺条件稳定可行,可用于工业生产。Objective To study the influence factors in the preparation process for phospholipids complex of total flavonoids from the leaves ofDiospyros kaki （PC-TF-LDK） and to optimize the conditions for the formation. Methods The solvent method was used to prepare the PC-TF-LDK, and the optimized conditions were obtained by means of single factor test and orthogonal design test with the complex ratio as evaluation criterion. The formation of the complex was analyzed by the X-ray diffraction and differential scanning calorimetry. Results The formation of the PC-TF-LDK was greatly influenced by the ratio of solvents, TF-LDK, and soybean phospholipid. The optimized preparation conditions for the PC-TF-LDK were obtained as follows： the solvent was anhydrous ethanol, the ratio of TF-LDK to soybean phospholipid was 1 ; 2, the mass of reaction concentration was 30 mg/mL, temperature was 30 ℃, and reaction time was 1 h. Under this condition, 96.95% complex ratio was achieved. Conclusion The preparation technology for PC-TF-LDK is reliable, stable, and available for industrial production.

关 键 词：柿叶黄酮 磷脂复合物 制备工艺 X-射线衍射 差示扫描量热 

分 类 号：R283.6[医药卫生—中药学]