Identification of Zinc Deficiency-Responsive MicroRNAs in Brassica juncea Roots by Small RNA Sequencing  

作　　者：SHI Dong-qing ZHANG Yuan MA Jin-hu LI Yu-long XU Jin 

机构地区：[1]Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences [2]College of Life Sciences,Henan University [3]College of Life Science and Geography,Qinghai Normal University [4]College of Agronomy,Shanxi Agricultural University [5]Key Laboratory of Agricultural Water Resources,Center for Agricultural Resources Research/Institute of Genetics and Developmental Biology,Chinese Academy of Sciences

出　　处：《Journal of Integrative Agriculture》2013年第11期2036-2044,共9页农业科学学报（英文版）

基　　金：supported by the Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2EW-Q-25);the National Natural Sciences Foundation of China(31170228;31272239);the Key Project of State Key Laboratory of Desert and Oasis Ecology;Xinjiang Institute of Ecology and Geography of Chinese Academy of Sciences;Hebei Province Natural Sciences Foundation for Distinguished Young Scientists;China (C2013503042)

摘　　要：The importance of zinc （Zn） as a micronutrient essential for plant growth and development is becoming increasingly apparent. Much of the world’s soil is Zn-deficient, and soil-based Zn deficiency is often accompanied by Zn deficiency in human populations. MicroRNAs （miRNAs） play important roles in the regulation of plant gene expression at the level of translation. Many miRNAs involved in the modulation of heavy metal toxicity responses in plants have been identiifed;however, the role of miRNAs in the plant Zn deifciency response is almost completely unknown. Using high-throughput Solexa sequencing, we identiifed several miRNAs that respond to Zn deifciency in Brassica juncea roots. At least 21 conserved candidate miRNA families, and 101 individual members within those families, were identiifed in both the control and the Zn-deifcient B. juncea roots. Among this, 15 miRNAs from 9 miRNA families were differentially expressed in the control and Zn-deifcient plants. Of the 15 differentially expressed miRNAs, 13 were up-regulated in the Zn-deifcient B. juncea roots, and only two, miR399b and miR845a, were down-regulated. Bioinformatics analysis indicated that these miRNAs were involved in modulating phytohormone response, plant growth and development, and abiotic stress responses in B. juncea roots. These data help to lay the foundation for further understanding of miRNA function in the regulation of the plant Zn deifciency response and its impact on plant growth and development.The importance of zinc （Zn） as a micronutrient essential for plant growth and development is becoming increasingly apparent. Much of the world’s soil is Zn-deficient, and soil-based Zn deficiency is often accompanied by Zn deficiency in human populations. MicroRNAs （miRNAs） play important roles in the regulation of plant gene expression at the level of translation. Many miRNAs involved in the modulation of heavy metal toxicity responses in plants have been identiifed;however, the role of miRNAs in the plant Zn deifciency response is almost completely unknown. Using high-throughput Solexa sequencing, we identiifed several miRNAs that respond to Zn deifciency in Brassica juncea roots. At least 21 conserved candidate miRNA families, and 101 individual members within those families, were identiifed in both the control and the Zn-deifcient B. juncea roots. Among this, 15 miRNAs from 9 miRNA families were differentially expressed in the control and Zn-deifcient plants. Of the 15 differentially expressed miRNAs, 13 were up-regulated in the Zn-deifcient B. juncea roots, and only two, miR399b and miR845a, were down-regulated. Bioinformatics analysis indicated that these miRNAs were involved in modulating phytohormone response, plant growth and development, and abiotic stress responses in B. juncea roots. These data help to lay the foundation for further understanding of miRNA function in the regulation of the plant Zn deifciency response and its impact on plant growth and development.

关 键 词：Zn deficiency Brassicajuncea microRNA deep sequencing 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]