花色苷对体外培养大鼠心肌细胞损伤的拮抗效应  

Antagonistic effect of Anthocyanin on injured myocardial cells in rats in vitro

作　　者：李玉白[1] 黄红焰[1] 

机构地区：[1]湖南环境生物职业技术学院,湖南衡阳421005

出　　处：《公共卫生与预防医学》2013年第6期14-16,共3页Journal of Public Health and Preventive Medicine

基　　金：衡阳市科技局计划项目(NO.2012KS72);南岳学者课题(201206)

摘　　要：目的观察花色苷对心肌细胞损伤的拮抗效应。方法设置3个实验组，即正常对照组，药物损伤组，花色苷组。选取出生1～3d的SD大鼠30只，将培养48h的心肌细胞制备成24瓶进行实验，随机分成3组。正常对照组不加任何药物，药物损伤组加入阿霉素ADM5mg／ml一共培养，花色苷组加入阿霉素60rain后加入10mg／L的花色苷。心肌细胞传代培养，测定各相关生化指标。结果与正常对照组比较，药物损伤组心肌肌钙蛋白T（cTnT）、心肌肌钙蛋白I（cTnI）、肌酸激酶（CK—MB）明显增高，药物损伤组总超氧化歧化酶（SOD）、CuZn一超氧化歧化酶（CuZn．SOD）和Mn一超氧化歧化酶（Mn—sot））水平均明显下降（P〈0．叭）；药物损伤组丙二醛（MDA）和NO含量明显增高（P〈0．01）；而花色苷组上述指标均有改善（P〈0．01），与正常对照组比较无统计学意义。结论花色苷对阿霉素造成的心肌损伤具有保护作用。Objective To study the protective effect of procyanidins on injured myocardial ceils. Methods Three experimental groups, namely normal control group, injured group, anthoeyanins treatment group were set up. Thirty 1-3day SD neonatal rats were selected. Myocardial cell preparation was cultured for 48 hours, and divided into 24 bottles for experiment. Three groups were randomly divided. Normal control group, without any drug, drug injury group, adriamycin ADM 5 mg/rrd was injected, anthocyanin group, adriamycin injection was given 60 minutes after adding 10 mg/L of anthocyanins. Under aseptic condition,cultured myocardial cells were subcuhured,and biochemical indexes were determined. Results Comparedwith the normal control group, in drug injured group ：cardiae troponin T （eTnT）, cardiac troponin I （eTn I） , ereatine kinase CK-MB increased significantly, while in the injured group ,the total superoxide dismutase（SOD） , CuZn- superoxide dismutase （CuZn -SOD ）and Mn- superoxide （Mn-SOD） dismutase all decreased significantly （P〈0. 01 ）; in drug injured group, MDA and NO contents increased significantly （P〈0.01） ; while in anthocyanin group ,both MDA and NO decreased （P〈0.01） ; and the indexes of Anthocyanins were improved （P〈0.01）. Conclusion Anthocyanin had protective effect on myocardial injury caused by adriamycin.

关 键 词：花色苷 心肌损伤 心肌肌钙蛋白T 心肌肌钙蛋白I 肌酸激酶 超氧化歧化酶 丙二醛 

分 类 号：R541[医药卫生—心血管疾病]