硝酸纤维素膜为HBsAg载体消毒试验法的研究  

STUDY ON METHOD OF DISINFECTION TEST USING CELLULOSE NITRATE MEMBRANE AS CARRIER OF HBsAg

作　　者：王雅贤[1] 陈清[1] 王红[1] 俞守义[1] 何海棠[2] 

机构地区：[1]第一军医大学流行病学教研室,广州510515 [2]广州第一军医大学附属南方医院传染科

出　　处：《中国消毒学杂志》2000年第4期237-240,共4页Chinese Journal of Disinfection

摘　　要：以 ４种消毒剂进行对硝酸纤维素膜上 ＨＢｓＡｇ消毒的研究。结果，对该膜上 ＨＢｓＡｇ的破坏效果，与悬液中 ＨＢｓＡｇ者一致。 ０．５％过氧乙酸、 ０．５％碘伏或 ２．０％碱性成二醛作用 ６０ ｍｉｎ，均可将效价≤１： ４０００的 ＨＢｓＡｇ破坏， ０．１％苯扎溴铵则不能。以硝酸纤维素膜作 ＨＢｓＡｇ载体，用免疫酶斑点法检测，结果直观，方法简便、易行，因ＨＢｓＡｇ吸附牢固，可用冲洗法去除残留消毒剂。Study on disinfection of HBsAg on cellulose nitrate membrane with four kinds of disinfectants was carried out. The results indicated that the efficacy of destruction of HBsAg on this membrane was same as that in suspension. 0.5% peracetic acid, 0.5% iodophor or 2.0% alkaline glutaraldehyde solution with a 60 minute contact time could destroy HBsAg at a titer ≤1: 400, while 0.1% benzalkonium bromide solution could not. When cellulose nitrate membrane was used as the carrier of HBsAg, examination with immunoenzyme spot method could give directly visible result. The method is simple and easy to be carried out. Because HBsAg is adsorbed firmly, the residual disinfectant can be removed by rinse.

关 键 词：乙型肝炎病毒 HBSAG 硝酸纤维素膜 消毒试验 

分 类 号：R187[医药卫生—流行病学] R512.62[医药卫生—公共卫生与预防医学]