亲代大鼠转Bt基因水稻暴露对其子代部分早期生理和神经发育指标的影响  

Effect of transgenic Bt rice TT51 on early physiological and neurological development of rats offspring

作　　者：王二辉 于洲 方海琴 汪会玲 刘海波 徐海滨 

机构地区：[1]国家食品安全风险评估中心,北京100021

出　　处：《中国食品卫生杂志》2013年第6期485-488,共4页Chinese Journal of Food Hygiene

基　　金：转基因生物新品种培育重大专项(NO.2012ZX08011001)

摘　　要：目的研究亲代大鼠转Bt基因水稻(TT51大米)暴露对其子代部分早期生理和神经发育指标的影响。方法亲代大鼠随机分为3组,每组雄性15只,雌性30只,分别给予按60%比例掺入市售大米、明恢63大米和TT51大米的饲料,连续喂养70 d后同组雄雌大鼠交配并产生子代,孕期和哺乳期各组母鼠继续给予相应受试大米的饲料。亲代大鼠每周称量和记录体重及食物消耗量,同时观察动物生长发育状况,观察和记录亲代雌鼠生殖指标。仔鼠出生后记录0、4、7、14及21 d体重,并观察仔鼠部分生理指标发育情况,同时进行听觉惊愕等神经发育指标的测定。断乳后,对各试验组部分仔鼠取脑等主要脏器进行病理检查。结果 TT51大米组与明恢63大米组和市售大米组比较,亲代大鼠一般毒性及生殖指标间差异均无统计学意义;子代部分生理发育指标和神经发育指标差异均无统计学意义;各试验组仔鼠脏器病理检查均未见有意义的变化。结论未见亲代大鼠转Bt基因水稻暴露对其子代早期部分生理和神经发育指标有不良影响。Objective To investigate the effects of parental expose to transgenic Bt rice TT51 on early physical and neurological development parameters of rats offspring. Methods Parental Wistar rats were fed with rice-based diets containing TT51, MingHui63 or grocery rice for 70 days prior mating, and females throughout pregnancy and lactation. Body weights of parental rats were measured weekly. Body weights of the pups on 0, 4, 7, 14 and 21d were measured and recorded. In each dietary group, pups were assessed for physical, neuromotor, reflexologic development postnatal. Results Compared with MingHui63 group and grocery rice group on physical, neuromotor and neurological reflex parameters in the offspring, no significant differences were observed in the TT51 rice group. Furthermore, no diet-related histopathological changes were observed. Conclusion The results of this study suggested that compared with parental rice exposure to transgenic Bt rice did not exert any effects on physical and neurological development indexes of rats offspring.

关 键 词：转BT基因水稻 生理发育 神经行为发育 转基因食品 食品安全 组织病理 毒理实验 

分 类 号：S511[农业科学—作物学] S865.12