薄荷油对雄性小鼠的抗生育作用研究  

Anti-fertility Effect of Peppermint Oil on Male Mice

作　　者：曹玫[1] 舒杨[2] 赵建[3] 李平[4] 李太强[4] 龙兴发 房俊[1] 殷中琼[2] 朱链发 

机构地区：[1]四川省医学科学院.四川省人民医院临床医学中心实验室,成都610072 [2]四川农业大学动物医学院,四川雅安625014 [3]四川大学生命科学学院,成都610064 [4]四川省甘孜州畜牧业科学研究所,四川康定626000

出　　处：《四川动物》2013年第6期908-911,F0003,共5页Sichuan Journal of Zoology

基　　金：公益性行业(农业)专项科研经费项目(201203041);四川省科技支撑计划(13ZC1635)

摘　　要：目的探讨薄荷油对雄性小鼠的抗生育作用。方法将60只健康的雄性性成熟昆明小鼠随机分成3个试验组和1个对照组,各试验组小鼠灌胃0.135 g/(kg·d),0.27 g/(kg·d)和0.54 g/(kg·d)的薄荷油0.5%羧甲基纤维素钠(sodium carboxymethylcellulose,CMC)混悬液,对照组灌胃0.5%羧甲基纤维素钠溶液,灌胃1周后按雌雄比2∶1合笼,继续对雄鼠灌胃至19 d,采集雄性小鼠的血液并测定血液生化指标,剖检灌胃雌雄小鼠,统计雌性小鼠的怀孕率,测定雄性小鼠睾丸与附睾的脏器系数及附睾精子活力,并固定睾丸和附睾,研究其组织学变化。结果与对照组相比较,雄性小鼠灌胃薄荷油后,导致雌性小鼠的怀孕率降低,睾丸脏器系数和附睾精子活率极显著降低(P<0.01),精子畸形率极显著升高(P<0.01)。光学显微镜下,试验组小鼠睾丸和附睾均有一定程度的损伤,睾丸间质减少,睾丸各级生精细胞排列疏松,曲细精管内有多核巨噬细胞浸润。附睾具正常的管腔结构,但能明显观察到管腔内精子减少。结论薄荷油对雄性小鼠具有抗生育作用,且主要通过影响精子的生成及破坏生精组织而达到,具有实际应用价值,可进一步开发成环境友好型鼠类抗生育药剂。Objective To investigate the anti-fertility effect of peppermint oil on male mice. Method 60 adult healthy male KM mice were randomly divided into three experiment groups and one control group. The mice of three experiment groups were orally administrated with peppermint oil mixed with 0.5% sodium carboxymethyl cellulose at the concentrations of 0. 135 g/（ kg . d）, 0.27 g/（ kg . d） and 0.54 g/（ kg . d）, respectively, and the mice in control group were orally administrated with 0.5% sodium earboxymethyl cellulose. After one week, adult female KM mice were lumped with experimental male mice at the ratio of 2：1, and the male mice were treated by peppermint oil till day 19. Finally, the blood of male mice was collected to determine the biochemical indices. The male and female mice were anatomized and the rate of pregnancy was observed. The organ coefficient of testis and epididymis, sperm survival rate and deformation rate, pathology and histology of the testis and epididymis tissues were studied. Results Compared with the control group, the pregnancy rate of peppermint oil treated male mice was decreased ; the organ coefficient of testis and sperm survival rate was declined, and the sperm deformation rate was significantly increased. Under the optical microscope, the testis and epididymis of peppermint oil treated male mice were damaged. The spermatogenic cells of testis were loosely arranged and the interstitial was reduced. The seminiferous tubules were infiltrated by maerophages. Although normal structure of epididymal duct was observed, there was hardly any sperm in the lumen. Conclusions The anti-fertility effect of peppermint oil can be used to in-hibit the spermatogenesis and destroy the spermatogenic tissue of male mice.

关 键 词：薄荷油 雄性小鼠 抗生育作用 

分 类 号：S853.7[农业科学—临床兽医学] S443[农业科学—兽医学]