7种消毒剂对鲟4种病原菌体外抑菌效果  

In vitro Inhibitory Effect of 7 Disinfectants on 4 Sturgeon-pathogenic Bacteria

作　　者：杨移斌[1] 夏永涛[2,3] 陈新[4] 邱军强[1] 曹海鹏[1] 胡鲲[1] 杨先乐[1] 

机构地区：[1]上海海洋大学,国家水生动物病原库,农业部淡水水产种质资源重点实验室,上海市水产养殖工程技术研究中心,上海高校知识服务平台上海海洋大学水产动物遗传育种中心(ZF1206),上海201306 [2]中国水产科学研究院,北京100039 [3]杭州千岛湖鲟龙科技股份有限公司,杭州310000 [4]中国水产科学研究院,东海水产研究所,上海200090

出　　处：《四川动物》2013年第6期912-915,921,共5页Sichuan Journal of Zoology

基　　金：国家重大科技成果转化项目(ZD-2012-345);公益性行业(农业)科研专项经费(201203085);国家863计划项目(2011AA10A216);国家自然科学基金资助项目(31172430);上海市科技兴农重点攻关项目(沪农科攻字(2011)第4-8号)

摘　　要：为了筛选高效的消毒剂用于防治鲟细菌性疾病，采用二倍稀释法测定了7种消毒剂对鲟4种病原菌的最小抑菌浓度（MIC）和最小杀菌质量浓度（MBC）。结果显示，高铁酸钾对鲟4种病原菌抑菌效果最好，MIC为8mg／L，MBC为8—16mg／L，戊二醛、甲醛、苯扎溴铵、过氧化氢及季铵盐络合碘5种消毒剂抑菌效果中等，聚维酮碘对鲟4种病原菌抑菌效果最差，聚维酮碘对鲟4种病原菌的MBC最大值为2048mg／L。根据上述研究结果，选择抑菌效果好的消毒剂高铁酸钾进一步研究了鲟4种病原菌随时间和高铁酸钾浓度变化的生长趋势，显示消毒液浓度为5mg／L、9mg／L的消毒液在短时间能抑制病原菌生长繁殖，病原菌数量同样能在短时间内繁殖恢复到原来的水平，浓度为13mg／L、17mg／L的高铁酸钾消毒液能快速杀灭病原菌，病原菌在较长时间维持在较低水平，甚至病原菌全部被杀死。研究对于使用高铁酸钾防治鲟养殖实践中的疾病具有重要的指导意义。To evaluate the bacteriostasis of different disinfectants on sturgeon-pathogenic bacteria, 7 kinds of disinfectants were tested by minimal inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） using double dilution method. The results showed that potassium ferrate had the best inhibitory effect on the 4 bacteria （MIC, 8 rag/L; MBC, 8 - 16 mg/L）, and 5 kinds of disinfectants including glutaraldebyde, formaldehyde, benzalkonium bromide, hydrogen peroxide and quaternary ammonium salt complexing iodine had moderate effects. On the contrary, the povidone iodine was failed to inhibit the growth of sturgeon-pathogenic bacteria. Subsequently, the inhibitory effects of different concentrations of potassium ferrate on four sturgeon-pathogenic bacteria were tested. The results showed that the potassium ferrate disinfectant could temporarily inhibit the growth of bacteria at the concentrations of 5 mg/L and 9 mg/L, and the number of bacteria would recover to the original level in a short time; wllen the concentrations of disinfectant were 13 mg/L and 17 mg/L, the growth rate of bacteria would be inhibited for a long time or even all totally killed. This study has an important guiding significance for the usage of potassium ferrate to treat the bacteria-related disease of sturgeon farming.

关 键 词：鲟 病原菌 消毒剂 抑菌效果 

分 类 号：S943[农业科学—水产养殖]